Document Detail

Increased expression of Toll-like receptor (TLR) 2 and TLR4 in the colonic mucosa of children with inflammatory bowel disease.
MedLine Citation:
PMID:  17991289     Owner:  NLM     Status:  MEDLINE    
Inflammatory bowel disease (IBD) may result from exaggerated stimulation of the mucosal immune system by luminal bacterial flora. Bacterial products are recognized by pattern recognition receptors such as Toll-like receptors (TLRs), which are key regulators of the innate immune system. Therefore, the expression of TLR2, TLR3 and TLR4 in colonic biopsy samples taken from children with active IBD were studied and compared to controls. Colonic biopsy samples were collected from macroscopically inflamed and non-inflamed regions of the mucosa of 12 children with freshly diagnosed IBD (fdIBD) and 23 children with relapsed IBD (rIBD). Specimens were also obtained from eight controls. TLR2, TLR3 and TLR4 mRNA expression and protein levels were determined by real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blot. We found higher TLR2 and TLR4 mRNA and protein levels in the inflamed colonic mucosa of children with fdIBD and rIBD compared to controls. In the non-inflamed colonic mucosa of children with fdIBD and rIBD, TLR2 and TLR4 mRNA and protein levels were similar to controls. TLR2 and TLR4 mRNA and protein levels also did not differ between children with fdIBD or rIBD in either inflamed or non-inflamed colonic mucosa. TLR3 mRNA expression and protein levels were similar in all groups studied. Our results of increased levels of TLR2 and TLR4 in the inflamed colonic mucosa of children with IBD confirm the hypothesis that innate immunity has an important role in the pathogenesis of this disease.
B Szebeni; G Veres; A Dezsõfi; K Rusai; A Vannay; M Mraz; E Majorova; A Arató
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-07
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  151     ISSN:  1365-2249     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-17     Completed Date:  2008-02-19     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  34-41     Citation Subset:  IM    
First Department of Pediatrics, Semmelweis University, Budapest, Hungary.
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MeSH Terms
Blotting, Western / methods
Case-Control Studies
Disease Susceptibility
Immunity, Innate
Inflammatory Bowel Diseases / immunology,  metabolism*
Intestinal Mucosa / immunology,  metabolism*
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Statistics, Nonparametric
Toll-Like Receptor 2 / analysis,  genetics,  metabolism*
Toll-Like Receptor 3 / analysis,  genetics,  metabolism
Toll-Like Receptor 4 / analysis,  genetics,  metabolism*
Reg. No./Substance:
0/RNA, Messenger; 0/TLR2 protein, human; 0/TLR3 protein, human; 0/TLR4 protein, human; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 3; 0/Toll-Like Receptor 4

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