| Increased expression of Toll-like receptor (TLR) 2 and TLR4 in the colonic mucosa of children with inflammatory bowel disease. | |
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MedLine Citation:
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PMID: 17991289 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inflammatory bowel disease (IBD) may result from exaggerated stimulation of the mucosal immune system by luminal bacterial flora. Bacterial products are recognized by pattern recognition receptors such as Toll-like receptors (TLRs), which are key regulators of the innate immune system. Therefore, the expression of TLR2, TLR3 and TLR4 in colonic biopsy samples taken from children with active IBD were studied and compared to controls. Colonic biopsy samples were collected from macroscopically inflamed and non-inflamed regions of the mucosa of 12 children with freshly diagnosed IBD (fdIBD) and 23 children with relapsed IBD (rIBD). Specimens were also obtained from eight controls. TLR2, TLR3 and TLR4 mRNA expression and protein levels were determined by real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blot. We found higher TLR2 and TLR4 mRNA and protein levels in the inflamed colonic mucosa of children with fdIBD and rIBD compared to controls. In the non-inflamed colonic mucosa of children with fdIBD and rIBD, TLR2 and TLR4 mRNA and protein levels were similar to controls. TLR2 and TLR4 mRNA and protein levels also did not differ between children with fdIBD or rIBD in either inflamed or non-inflamed colonic mucosa. TLR3 mRNA expression and protein levels were similar in all groups studied. Our results of increased levels of TLR2 and TLR4 in the inflamed colonic mucosa of children with IBD confirm the hypothesis that innate immunity has an important role in the pathogenesis of this disease. |
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Authors:
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B Szebeni; G Veres; A Dezsõfi; K Rusai; A Vannay; M Mraz; E Majorova; A Arató |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-07 |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 151 ISSN: 1365-2249 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2007-12-17 Completed Date: 2008-02-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: England |
Other Details:
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Languages: eng Pagination: 34-41 Citation Subset: IM |
Affiliation:
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First Department of Pediatrics, Semmelweis University, Budapest, Hungary. szebeni@gyer1.sote.hu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Blotting, Western / methods Case-Control Studies Child Colon* Disease Susceptibility Female Humans Immunity, Innate Inflammatory Bowel Diseases / immunology, metabolism* Intestinal Mucosa / immunology, metabolism* Male RNA, Messenger / analysis Recurrence Reverse Transcriptase Polymerase Chain Reaction Statistics, Nonparametric Toll-Like Receptor 2 / analysis, genetics, metabolism* Toll-Like Receptor 3 / analysis, genetics, metabolism Toll-Like Receptor 4 / analysis, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/TLR2 protein, human; 0/TLR3 protein, human; 0/TLR4 protein, human; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 3; 0/Toll-Like Receptor 4 |
| Comments/Corrections | |
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