Document Detail


Increased expression of TRPV1 in squamous cell carcinoma of the human tongue.
MedLine Citation:
PMID:  19320840     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Recent reports have unambiguously identified the presence and the growth-modulatory role of transient receptor potential vanilloid-1 (TRPV1), a central integrator of pain sensation, on numerous non-neuronal cell types and, of great importance, in certain malignancies. In this study, we have investigated the molecular expression of TRPV1 in the human tongue and its high-incidence malignant (squamous cell carcinoma, SCC) and premalignant (leukoplakia) conditions. METHODS: Immunohistochemistry, Western blotting and quantitative 'real-time' Q-PCR were performed to define the expression of TRPV1. RESULTS: A weak and sparse TRPV1-specific immunoreactivity was identified in the basal layers of the healthy human tongue epithelium. By contrast, we observed a dramatically elevated TRPV1-immunoreactivity in all layers of the epithelium both in precancerous and malignant samples. Furthermore, statistical analysis revealed that the marked overexpression of TRPV1 found in all grades of SCC showed no correlation with the degree of malignancy of the tumours. Finally, the molecular expression of TRPV1 was also identified in an SCC-derived cell line and was shown to be increased in parallel with the accelerated growth of the cells. CONCLUSION: Collectively, our findings identify TRPV1 as a novel, promising target molecule in the supportive treatment and diagnosis of human tongue SCC.
Authors:
R Marincsák; B I Tóth; G Czifra; I Márton; P Rédl; I Tar; L Tóth; L Kovács; T Bíró
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-23
Journal Detail:
Title:  Oral diseases     Volume:  15     ISSN:  1601-0825     ISO Abbreviation:  Oral Dis     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-15     Completed Date:  2009-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508565     Medline TA:  Oral Dis     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  328-35     Citation Subset:  D    
Affiliation:
Department of Physiology, University of Debrecen, Medical and Health Science Centre, Research Centre for Molecular Medicine, Debrecen, Hungary.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Blotting, Western
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Epithelial Cells / pathology
Female
Humans
Image Processing, Computer-Assisted
Immunohistochemistry
Leukoplakia, Oral / pathology
Male
Middle Aged
Polymerase Chain Reaction / methods
TRPV Cation Channels / analysis*
Tongue / pathology
Tongue Neoplasms / pathology*
Tumor Markers, Biological / analysis
Chemical
Reg. No./Substance:
0/TRPV Cation Channels; 0/TRPV1 protein, human; 0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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