| Increased expression of Nlp, a potential oncogene in ovarian cancer, and its implication in carcinogenesis. | |
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MedLine Citation:
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PMID: 18538832 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Nlp (Ninein-like protein), a novel centrosome protein involved in microtubule nucleation, has been studied extensively in our laboratory, and its overexpression has been found in some human tumors. To understand the role of Nlp in human ovarian cancer development, we studied the correlation of Nlp expression with clinicopathological parameters and survival in epithelial ovarian cancer, and the impact of Nlp overexpression on ovarian cancer cells. METHODS: Nlp expression in normal, borderline, benign and malignant epithelial ovarian tissues was examined by immunohistochemistry. The correlation between Nlp expression and tumor grade, FIGO stage and histological type was also evaluated. Survival was calculated using Kaplan-Meier estimates. Cell proliferation and apoptosis were assayed after stable transfection of pEGFP-C3-Nlp or empty vector in human ovarian cancer cell line SKOV3. RESULTS: Nlp was positive in 1 of 10 (10%) normal ovarian tissues, 5 of 34 (14.7%) benign tumors, 9 of 26 (34.6%) borderline tumors and 73 of 131 (56.0%) ovarian tumors. Nlp immunoreactivity intensity significantly correlated with tumor grade, but not with FIGO stage or histological type. Kaplan-Meier curves showed that Nlp overexpression was marginally associated with decreased overall survival. Overexpression of Nlp enhanced proliferation and inhibited apoptosis induced by paclitaxel in the SKOV3 cell line. CONCLUSIONS: Overexpression of Nlp in ovarian tumors raises the possibility that Nlp may play a role in ovarian carcinogenesis. |
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Authors:
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Danni Qu; Hongyan Qu; Ming Fu; Xuelian Zhao; Rong Liu; Lihua Sui; Qimin Zhan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-06 |
Journal Detail:
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Title: Gynecologic oncology Volume: 110 ISSN: 1095-6859 ISO Abbreviation: Gynecol. Oncol. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-22 Completed Date: 2008-08-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0365304 Medline TA: Gynecol Oncol Country: United States |
Other Details:
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Languages: eng Pagination: 230-6 Citation Subset: IM |
Affiliation:
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The Tumor Hospital of Harbin Medical University, Heilongjiang Province, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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genetics,
metabolism*,
pathology Antineoplastic Agents, Phytogenic / pharmacology Apoptosis / drug effects, physiology Cell Growth Processes / physiology Cell Line, Tumor Cell Transformation, Neoplastic / genetics, metabolism* Female Humans Immunohistochemistry Microtubule-Associated Proteins / biosynthesis*, genetics Middle Aged Neoplasm Staging Nuclear Proteins / biosynthesis*, genetics Ovarian Neoplasms / drug therapy, genetics, metabolism*, pathology Paclitaxel / pharmacology Survival Rate Transfection |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Microtubule-Associated Proteins; 0/NIp protein, human; 0/Nuclear Proteins; 33069-62-4/Paclitaxel |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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