Document Detail


Increased expression of Ia antigens on B cells after neonatal induction of lymphoid chimerism in mice: role of interleukin 4.
MedLine Citation:
PMID:  2138556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BALB/c mice rendered chimeric at birth by injection of 10(8) (A/J X BALB/c)F1 spleen cells develop a lupus-like autoimmune disease linked to the activation of donor B cells by host T cells. As in vitro studies previously indicated that interleukin 4 (IL4) was a mediator of the interactions between T and B cells, we analyzed the intensity of Ia antigen expression on B cells of chimeric mice. Flow cytometric analysis with anti-Ia monoclonal antibodies (mAb) revealed that B cells from spleens and lymph nodes of 2-week-old chimeric BALB/c mice displayed a two- to threefold increase in membrane Ia antigen expression, this increase still being present in spleens of 30-week-old animals. An increase in Ia antigen expression was also found in the small number of donor B cells detected in spleens and lymph nodes of chimeric mice. IL4 was the major stimulus leading to increased B cell Ia antigen expression, as this phenomenon was substantially prevented by in vivo treatment of chimeric mice with the anti-IL4 11B11 mAb. In vitro experiments revealed that host splenic T cells of chimeric mice, while unable to generate anti-donor cytotoxic T lymphocytes, secreted significant amounts of IL 4 when stimulated in mixed lymphocyte cultures (MLC) with donor alloantigens. This IL4 secretion led to an increased expression of Ia antigens on donor-type F1 B cells present in MLC. No significant increase in Ia antigen expression was found on syngeneic BALB/c B cells co-cultured with T cells from chimeric mice unless A/J B cells were added to the cultures. Taken together, these findings indicate that increased Ia antigen expression on donor B cells is induced by IL4 secreted by anti-donor T cells. IL4 released in this setting also leads to increased Ia antigen expression on host B cells through a bystander effect.
Authors:
D Abramowicz; J M Doutrelepont; P Lambert; P Van der Vorst; C Bruyns; M Goldman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  20     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-05-03     Completed Date:  1990-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  469-76     Citation Subset:  IM    
Affiliation:
Laboratoire Pluridisciplinaire de Recherche Expérimentale Biomédicale, Cliniques Universitaires de Bruxelles, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / immunology
B-Lymphocytes / immunology*
Chimera / immunology*
Flow Cytometry
Histocompatibility Antigens Class II / immunology*
Immune Tolerance
Interleukin-4 / physiology*
Lymph Nodes / immunology
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred Strains
Spleen / immunology
T-Lymphocytes / immunology
Chemical
Reg. No./Substance:
0/Histocompatibility Antigens Class II; 207137-56-2/Interleukin-4

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