Document Detail


Increased excitability and spontaneous activity of rat sensory neurons following in vitro stimulation of sympathetic fiber sprouts in the isolated dorsal root ganglion.
MedLine Citation:
PMID:  20800969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many chronic pain conditions including complex regional pain syndrome are exacerbated by sympathetic activity. In animal models, sympathetic fibers sprout into the dorsal root ganglia (DRG) after peripheral nerve injury, forming abnormal connections with sensory neurons. However, functional studies of sympathetic-sensory connections have been limited largely to in vivo studies. This study describes a new method for studying sympathetic-sensory connections in an isolated whole DRG preparation in the rat spinal nerve ligation (SNL) model. Three days after ligation of the ventral ramus of the spinal nerve (SNL), sympathetic fibers sprouting into the DRG were observed to originate largely in the intact dorsal ramus of the spinal nerve, which at the lumbar level is a small branch of the spinal nerve separating from the ventral ramus near the intervertebral foramen. In whole DRG isolated 3 days after SNL, microelectrode recordings of sensory neurons showed that repeated stimulation of the dorsal ramus enhanced spontaneous activity in large and medium diameter neurons and reduced rheobase in large neurons. These effects, which were slow and long lasting, were attributed to stimulation of the sympathetic sprouts because: stimulation had no effect in uninjured DRG; and effects could be reduced or eliminated by a "cocktail" of antagonists of norepinephrine and ATP receptors, by pretreatment with the sympathetic release blocker bretylium, or by pre-cutting the grey ramus through which sympathetic fibers coursed to the ligated DRG. The latter treatment, a relatively minimal form of sympathectomy, was also highly effective in reducing mechanical pain ipsilateral to the SNL.
Authors:
Wenrui Xie; Judith A Strong; Jun-Ming Zhang
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-08-30
Journal Detail:
Title:  Pain     Volume:  151     ISSN:  1872-6623     ISO Abbreviation:  Pain     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-15     Completed Date:  2011-02-14     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  7508686     Medline TA:  Pain     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  447-59     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic Fibers / physiology*
Animals
Disease Models, Animal
Electric Capacitance
Ganglia, Spinal / pathology*
Hyperalgesia / physiopathology
Male
Membrane Potentials / physiology
Neurons, Afferent / physiology*
Pain Threshold / physiology
Peripheral Nervous System Diseases / pathology*
Phosphopyruvate Hydratase / metabolism
Physical Stimulation / methods
Rats
Rats, Sprague-Dawley
Time Factors
Tyrosine 3-Monooxygenase / metabolism
Grant Support
ID/Acronym/Agency:
NS45594/NS/NINDS NIH HHS; NS55860/NS/NINDS NIH HHS; R01 NS045594/NS/NINDS NIH HHS; R01 NS055860/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
EC 1.14.16.2/Tyrosine 3-Monooxygenase; EC 4.2.1.11/Phosphopyruvate Hydratase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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