Document Detail


Increased endogenous ascorbyl free radical formation with singlet oxygen scavengers in reperfusion injury: an EPR and functional recovery study in rat hearts.
MedLine Citation:
PMID:  11156483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to investigate the effect of singlet oxygen ((1)O2) scavengers on functional recovery and ascorbyl free radical (AFR) formation in isolated ischemic rat hearts. Hearts were subjected to 40 min. of global ischemia followed by 30 min. of reperfusion. Hemodynamics were measured as heart rate (HR), coronary flow (CF), left ventricular developed pressure (LVDP) and contractility (dP/dt). Electron paramagnetic resonance (EPR) spectroscopy was used to measure AFR release in coronary perfusate during the first two min. of reperfusion as a function of ROS scavengers. Relative to ischemic controls the administration of the (1)O2 scavengers 2,2,6,6-tetramethyl-4-piperidone x HCl (4-oxo-TEMP), carnosine (beta-alanyl-L-histidine) or a combination of the two significantly improved functional recovery as measured by LVDP. While no AFR signal was detected in coronary perfusate collected during preischemic perfusion with and without (1)O2 scavengers, the AFR background signal due to ischemia was significantly increased with the (1)O2 and *O2- scavengers. No such increase was observed with the hydroxyl radical (*OH) scavenger mannitol. Besides the AFR increase with the (1)O2 and *O2- scavengers the functional recovery was only significantly improved with the (1)O2 scavengers. In contrast to previous AFR studies we found with endogenous AFR that an increased AFR formation is not necessarily only reflecting increased oxidative stress but can also report improved functional recovery. Combining the hemodynamic data with increased AFR formation in the presence of several different ROS scavengers gives supportive evidence for (1)O2 also being involved in reperfusion injury.
Authors:
J W Lee; E V Bobst; Y G Wang; M M Ashraf; A M Bobst
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular and molecular biology (Noisy-le-Grand, France)     Volume:  46     ISSN:  0145-5680     ISO Abbreviation:  Cell. Mol. Biol. (Noisy-le-grand)     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-11     Completed Date:  2001-03-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9216789     Medline TA:  Cell Mol Biol (Noisy-le-grand)     Country:  France    
Other Details:
Languages:  eng     Pagination:  1383-95     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Cincinnati, Medical Center, OH, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascorbic Acid / metabolism*
Blood Flow Velocity / drug effects
Blood Pressure / drug effects
Carnosine / pharmacology
Diuretics, Osmotic / pharmacology
Electron Spin Resonance Spectroscopy / methods*
Free Radical Scavengers / pharmacology
Free Radicals
Heart / drug effects
Heart Rate / drug effects
Male
Mannitol / pharmacology
Models, Biological
Models, Chemical
Myocardium / metabolism*
Oxygen / metabolism*
Piperidones / pharmacology
Rats
Rats, Sprague-Dawley
Reperfusion Injury / metabolism*
Superoxide Dismutase / pharmacology
Time Factors
Tranquilizing Agents / pharmacology
Triacetoneamine-N-Oxyl / analogs & derivatives*,  pharmacology
Grant Support
ID/Acronym/Agency:
HL23597/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Diuretics, Osmotic; 0/Free Radical Scavengers; 0/Free Radicals; 0/Piperidones; 0/Tranquilizing Agents; 2896-70-0/Triacetoneamine-N-Oxyl; 305-84-0/Carnosine; 50-81-7/Ascorbic Acid; 69-65-8/Mannitol; 7782-44-7/Oxygen; 826-36-8/tempidon; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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