| Increased efficiency of fluorescence in situ hybridization (FISH) using the microwave. | |
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MedLine Citation:
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PMID: 15923358 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new method is described for performing fluorescence in situ hybridization (FISH). FISH signals are enhanced by microwave pulses applied during the DNA-DNA hybridization process. It is the first description of FISH with a single/low-copy probe done more efficiently by application of microwave; the latter leads to quick results or enhancement of weak signals. Microwave FISH has been compared systematically with normal FISH, and we could demonstrate the efficiency of microwave irradiation especially in the first 100 min of hybridization. |
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Authors:
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Anja Weise; Thomas Liehr; Uwe Claussen; Karl-Jürgen Halbhuber |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-05-27 |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 53 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-03 Completed Date: 2005-10-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 1301-3 Citation Subset: IM |
Affiliation:
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Institut für Humangenetik und Anthropologie, Jena, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosome Aberrations Chromosomes, Human, Pair 1 DNA / chemistry Female Humans In Situ Hybridization, Fluorescence / methods* Microwaves* Prenatal Diagnosis / methods |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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