Document Detail


Increased efficiency of fluorescence in situ hybridization (FISH) using the microwave.
MedLine Citation:
PMID:  15923358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new method is described for performing fluorescence in situ hybridization (FISH). FISH signals are enhanced by microwave pulses applied during the DNA-DNA hybridization process. It is the first description of FISH with a single/low-copy probe done more efficiently by application of microwave; the latter leads to quick results or enhancement of weak signals. Microwave FISH has been compared systematically with normal FISH, and we could demonstrate the efficiency of microwave irradiation especially in the first 100 min of hybridization.
Authors:
Anja Weise; Thomas Liehr; Uwe Claussen; Karl-Jürgen Halbhuber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-05-27
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  53     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-03     Completed Date:  2005-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1301-3     Citation Subset:  IM    
Affiliation:
Institut für Humangenetik und Anthropologie, Jena, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Aberrations
Chromosomes, Human, Pair 1
DNA / chemistry
Female
Humans
In Situ Hybridization, Fluorescence / methods*
Microwaves*
Prenatal Diagnosis / methods
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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