Document Detail


Increased cyclooxygenase-2 expression in human squamous cell carcinomas of the head and neck and inhibition of proliferation by nonsteroidal anti-inflammatory drugs.
MedLine Citation:
PMID:  12174888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Cyclooxygenase-2 (COX-2) has been found to be up-regulated in several types of human cancers and its role in the carcinogenic process has been proposed The aim of this study was to examine the expression of COX-2 in human squamous cell carcinoma of the head and neck (SCCHN) and to find out the effects of COX-2 inhibitors on the growth of cultured cells. MATERIALS AND METHODS: We investigated the effect of indomethacin and NS-398 at various concentrations on the growth of SCCHN cell lines using cell proliferation assay, cell cycle analysis and quantification of apoptosis. RESULTS: Immunostaining revealed a significantly increased COX-2 expression in tumor tissues compared with normal controls (p<0.05). Western blotting analysis using a COX-2 antibody, indicated that seven SCCHN cell lines tested constitutively expressed COX-2 protein. Treatment of head and neck cancer cells with NS-398 (10-200 microM) or indomethacin (50-1000 microM) for 72 hours showed a significant dose-dependent inhibition of cell growth (p<0.01) and a significant increase in the number of cells in the G0/G1-phases of the cell cycle with a concomitant reduction at the S-phase in a dose-dependent manner (p<0.05). NS-398 was more effective in cell cycle arrest and growth inhibition than indomethacin (p<0.05) and induced significant apoptosis in two out of three SCCHN cell lines tested at the concentration of 100 microM. CONCLUSION: Our study showed that COX-2 could be a participant in carcinogenesis of SCCHN and that COX-2 inhibitors would be a potential tool for the treatment and prevention of SCCHN.
Authors:
Dong W Lee; Myung-Whun Sung; Seok-Woo Park; Weon-Jin Seong; Jong-Lyel Roh; Bumjung Park; Dae-Seog Heo; Kwang H Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  22     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2002 Jul-Aug
Date Detail:
Created Date:  2002-08-14     Completed Date:  2002-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2089-96     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Korea.
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MeSH Terms
Descriptor/Qualifier:
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Apoptosis / drug effects
Carcinoma, Squamous Cell / drug therapy,  enzymology,  genetics*,  pathology
Cell Cycle / drug effects
Cell Division / drug effects
Cyclooxygenase 2
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Neoplastic / drug effects
Head and Neck Neoplasms / drug therapy,  enzymology,  genetics*,  pathology
Humans
Immunohistochemistry
Indomethacin / pharmacology
Isoenzymes / analysis,  genetics*
Membrane Proteins
Nitrobenzenes / pharmacology
Prostaglandin-Endoperoxide Synthases / analysis,  genetics*
Sulfonamides / pharmacology
Tumor Markers, Biological / analysis
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Isoenzymes; 0/Membrane Proteins; 0/Nitrobenzenes; 0/Sulfonamides; 0/Tumor Markers, Biological; 123653-11-2/N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; 53-86-1/Indomethacin; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

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