Document Detail

Increased cyclic AMP content directly correlated with morphological transformation of cells infected with a temperature-sensitive mutant of mouse sarcoma virus.
MedLine Citation:
PMID:  6252097     Owner:  NLM     Status:  MEDLINE    
Normal rat kidney cells infected with a cold-sensitive mutant of mouse sarcoma virus [NRK(MSV-lb)] morphologically transform when exposed to adenosine 3':5' cyclic monophosphate (cAMP) at the restrictive temperature. The cAMP-induced morphological changes occur rapidly and are reversible. Agents capable of elevating endogenous levels of cAMP [prostaglandin E1 (PGE1) and cholera toxin (CT)] induced morphological transformation of NRK(MSV-lb) cells at the restrictive temperature that was concentration dependent, potentiated by cAMP phosphodiesterase inhibitors, and not prevented by inhibitors of DNA, RNA, and protein synthesis. Prostaglandin E1 stimulated a transient increase in the intracellular level of cAMP with a concomitant morphological transformation and reversion of cells as cAMP levels decline. The maximum increase is reached by 10 min, followed by a decline to near basal level by 80 min. In contrast, incubation of cells with CT resulted in irreversible morphological transformation and increased levels of cAMP first detectable by 1 hr with maximum levels reached by 24 hr. Heated CT (100 degrees C, 20 min) was without effect. Addition of CT to reverted PGE1-treated cells resulted in morphological transformation suggesting the existence of discrete receptors in NRK(MSV-lb) cells.
K D Somers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro     Volume:  16     ISSN:  0073-5655     ISO Abbreviation:  In Vitro     Publication Date:  1980 Oct 
Date Detail:
Created Date:  1980-12-18     Completed Date:  1980-12-18     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0063733     Medline TA:  In Vitro     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  851-8     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Transformation, Neoplastic*
Cell Transformation, Viral*
Cholera Toxin / pharmacology
Cyclic AMP / metabolism*
Prostaglandins E / pharmacology
Sarcoma Viruses, Murine / physiology*
Grant Support
Reg. No./Substance:
0/Prostaglandins E; 60-92-4/Cyclic AMP; 9012-63-9/Cholera Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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