| Increased connexin 43 expression improves the migratory and proliferative ability of H9c2 cells by Wnt-3a overexpression. | |
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MedLine Citation:
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PMID: 17558443 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The change of connexin 43 (Cx43) expression and the biological behaviors of Cx43 in rat heart cell line H9c2, expressing Wnt-3a (wingless-type MMTV integration site family, member 3A), were evaluated in the present study. Plasmid pcDNA3.1/Wnt-3a was constructed and transferred into H9c2 cells. The cell model Wnt-3a(+)-H9c2 steadily expressing Wnt-3a was obtained. Compared with H9c2 and pcDNA3.1-H9c2 cells, the expression of Cx43 in Wnt-3a(+)-H9c2 cells was clearly increased, the proliferation of Wnt-3a(+)-H9c2 cells was significantly changed, and cell migration abilities were also improved(P<0.05). In comparison with H9c2 and pcDNA3.1-H9c2 cells, the G2 phase of the cell cycle increased by 11% in Wnt-3a(+)-H9c2 cells. Thus, Wnt-3a overexpression is associated with an increase in Cx43 expression and altered migratory and proliferative activity in H9c2 cells. Cx43 might be one of the downstream target genes regulated by Wnt-3a. |
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Authors:
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Xiaoyu Liu; Wen Liu; Ling Yang; Beili Xia; Jinyan Li; Ji Zuo; Xiaotian Li |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta biochimica et biophysica Sinica Volume: 39 ISSN: 1672-9145 ISO Abbreviation: Acta Biochim. Biophys. Sin. (Shanghai) Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-11 Completed Date: 2007-08-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101206716 Medline TA: Acta Biochim Biophys Sin (Shanghai) Country: China |
Other Details:
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Languages: eng Pagination: 391-8 Citation Subset: IM |
Affiliation:
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Department of Cellular and Genetic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / physiology Cell Differentiation / physiology Cell Movement / physiology* Cell Proliferation* Cells, Cultured Cloning, Molecular Connexin 43 / metabolism* Gap Junctions / genetics Gene Expression / genetics* Myocardium / cytology Rats Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Trans-Activators / metabolism* Wnt Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Connexin 43; 0/Trans-Activators; 0/Wnt Proteins; 0/Wnt-3 protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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