Document Detail


Increased concentrations of angiotensin-converting enzyme in the intimal hyperplasia of experimental vein grafts.
MedLine Citation:
PMID:  7516009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Local renin and angiotensin-converting enzyme (ACE) activity were recently implicated in development of intimal hyperplasia after vascular injury, but little is known about the local responses of angiotensin I/II (AI/AII) and local ACE activity in vein graft physiology. The activity of the local ACE system of experimental vein grafts was examined in this study. The right carotid artery was divided and bypassed in 21 New Zealand White rabbits, using the right external jugular vein. The left external jugular vein was used as a control. Veins and vein grafts were harvested after 14 days. Rings from both vessels were studied in vitro under isometric tension, and dose-response curves to AI and AII were obtained. AI responses were also measured in the presence of captopril. The tissue concentrations of ACE in both vessels were estimated by spectrophotometry and were localized by immunohistochemistry. The responses of the veins to AI and AII were multiphasic, whereas the responses of vein grafts were sigmoid-shaped. Incubation of vein grafts with captopril significantly decreased the sensitivity to AI (p < 0.0001). Immunohistochemical localization identified ACE in the endothelial layer of the veins and vein grafts, but also at a greater density in the intimal hyperplasia of the vein graft. The concentration of ACE was 1.92 +/- 0.16 U/g (wet weight; mean +/- SEM, n = 9) in vein grafts and 1.39 +/- 0.05 U/g in the veins (38% increase, p < 0.05, n = 9).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
M K O'Donohoe; M G Davies; Z S Radic; E M Mikat; P O Hagen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  23     ISSN:  0160-2446     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-07-08     Completed Date:  1994-07-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  594-601     Citation Subset:  IM    
Affiliation:
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carotid Arteries / surgery
Hyperplasia
Immunohistochemistry
Male
Peptidyl-Dipeptidase A / metabolism*
Rabbits
Renin / metabolism
Tunica Intima / enzymology,  pathology*
Veins / enzymology,  pathology,  transplantation*
Grant Support
ID/Acronym/Agency:
HL 15448/HL/NHLBI NIH HHS; TW 04810/TW/FIC NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.15.1/Peptidyl-Dipeptidase A; EC 3.4.23.15/Renin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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