Document Detail

Increased c-myb mRNA expression in Barrett's esophagus and Barrett's-associated adenocarcinoma.
MedLine Citation:
PMID:  11469901     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Esophageal adenocarcinoma develops through a multistage process which is characterized histopathologically by progression from Barrett's intestinal metaplasia to Barrett's esophagus with dysplasia and ultimately to adenocarcinoma. The genetic basis of this process is increasingly well understood, but no studies have examined the role of the transcription factor c-myb in this disease. MATERIALS AND METHODS: c-myb mRNA expression levels were measured using a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method in specimens of Barrett's intestinal metaplasia (n = 16), adenocarcinoma (n = 22), matching normal squamous esophagus tissues (n = 38), and normal squamous esophagus tissues from patients without Barrett's esophagus or chronic gastroesophageal reflux disease (n = 10). RESULTS: The median c-myb mRNA expression levels were significantly increased in Barrett's intestinal metaplasia tissues compared to normal esophagus tissues (P = 0.013) and in Barrett's-associated adenocarcinoma tissues compared to normal squamous esophagus tissues (P = 0.001). The c-myb expression levels increased progressively and significantly in histopathologically worse tissue types, with an increase from normal squamous esophagus mucosa to Barrett's intestinal metaplasia, and from Barrett's intestinal metaplasia to adenocarcinoma of the esophagus (P = 0.002). Median c-myb expression levels were also significantly higher in histologically normal squamous esophagus tissues from cancer patients compared to normal esophagus tissues from patients without cancer (P < 0.001) and a control group without evidence of Barrett's esophagus or gastroesophageal reflux disease (P = 0.003). Very high c-myb mRNA expression levels were found only in patients with cancer. CONCLUSION: These findings suggest that upregulation of c-myb mRNA expression is an early event in the development of Barrett's esophagus and associated adenocarcinoma, that high c-myb mRNA expression levels may be a clinically useful biomarker for the detection of occult adenocarcinoma, and that a widespread cancer "field" effect is present in the esophagus of patients with Barrett's-associated adenocarcinoma.
J Brabender; R V Lord; K D Danenberg; R Metzger; P M Schneider; J M Park; D Salonga; S Groshen; D D Tsao-Wei; T R DeMeester; A H Hölscher; P V Danenberg
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  99     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-07-25     Completed Date:  2001-08-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  301-6     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Biochemistry and Molecular Biology, USC/Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, 1441 Eastlake Avenue, Los Angeles, CA 90033, USA.
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MeSH Terms
Adenocarcinoma / physiopathology*
Barrett Esophagus / physiopathology*
Esophageal Neoplasms / physiopathology*
Gene Expression Regulation, Neoplastic*
Middle Aged
Proto-Oncogene Proteins c-myb / genetics*
RNA, Messenger / analysis
Reg. No./Substance:
0/Proto-Oncogene Proteins c-myb; 0/RNA, Messenger

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