Document Detail


Increased bone resorption and impaired bone microarchitecture in short-term and extended high-fat diet-induced obesity.
MedLine Citation:
PMID:  20171704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although obesity traditionally has been considered a condition of low risk for osteoporosis, this classic view has recently been questioned. The aim of this study was to assess bone microarchitecture and turnover in a mouse model of high-fat diet-induced obesity. Seven-week-old male C57BL/6J mice (n = 18) were randomized into 3 diet groups. One third (n = 6) received a low-fat diet for 24 weeks, one third was kept on an extended high-fat diet (eHF), and the remaining was switched from low-fat to high-fat chow 3 weeks before sacrifice (sHF). Serum levels of insulin, leptin, adiponectin, osteocalcin, and cross-linked telopeptides of type I collagen (CTX) were measured. In addition, bone microarchitecture was analyzed by micro-computed tomography; and lumbar spine bone density was assessed by dual-energy x-ray absorptiometry. The CTX, body weight, insulin, and leptin were significantly elevated in obese animals (sHF: +48%, +24%, +265%, and +102%; eHF: +43%, +52%, +761%, and +292%). The CTX, body weight, insulin, and leptin showed a negative correlation with bone density and bone volume. Interestingly, short-term high-fat chow caused similar bone loss as extended high-fat feeding. Bone volume was decreased by 12% in sHF and 19% in eHF. Bone mineral density was 25% (sHF) and 27% (eHF) lower when compared with control mice on low-fat diet. As assessed by the structure model index, bone microarchitecture changed from plate- to rod-like appearance upon high-fat challenge. Trabecular and cortical thickness remained unaffected. Short-term and extended high-fat diet-induced obesity caused significant bone loss in male C57BL/6J mice mainly because of resorptive changes in trabecular architecture.
Authors:
Janina M Patsch; Florian W Kiefer; Peter Varga; Pamela Pail; Martina Rauner; Daniela Stupphann; Heinrich Resch; Doris Moser; Philippe K Zysset; Thomas M Stulnig; Peter Pietschmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-20
Journal Detail:
Title:  Metabolism: clinical and experimental     Volume:  60     ISSN:  1532-8600     ISO Abbreviation:  Metab. Clin. Exp.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-24     Completed Date:  2011-03-04     Revised Date:  2011-04-06    
Medline Journal Info:
Nlm Unique ID:  0375267     Medline TA:  Metabolism     Country:  United States    
Other Details:
Languages:  eng     Pagination:  243-9     Citation Subset:  IM    
Copyright Information:
© 2011 Elsevier Inc. All rights reserved.
Affiliation:
Division of Cellular and Molecular Pathophysiology, Department of Pathophysiology, Center of Physiology, Pathophysiology and Immunology, Medical University Vienna, A-1090 Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Absorptiometry, Photon
Adiponectin / blood
Adipose Tissue / metabolism
Animals
Bone Density / drug effects*
Bone Resorption / metabolism*,  radiography
Bone and Bones / metabolism,  radiography,  ultrastructure*
Collagen Type I / analysis
Diet, Fat-Restricted
Dietary Fats / administration & dosage*
Insulin / blood
Leptin / blood
Lumbar Vertebrae / metabolism,  radiography,  ultrastructure
Male
Mice
Mice, Inbred C57BL
Obesity / etiology*
Osteocalcin / blood
Grant Support
ID/Acronym/Agency:
P 20239-B13//Austrian Science Fund FWF
Chemical
Reg. No./Substance:
0/Adiponectin; 0/Collagen Type I; 0/Dietary Fats; 0/Leptin; 104982-03-8/Osteocalcin; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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