| Increased blood levels of human S100 in melanoma chick embryo xenografts' circulation. | |
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MedLine Citation:
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PMID: 11277496 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The chorioallantoic membrane (CAM) of the fertilized egg allows grafting of human melanomas for short-term investigations and offers the opportunity to investigate the behavior of metastasizing cells and the release of S100beta into peripheral blood. Tissue from one primary melanoma as well as cutaneous and subcutaneous metastases of 10 melanoma patients with elevated levels of S100 in the peripheral blood before surgery were transplanted onto the CAM of chick embryos at day 5/6 of development. Grafts were nourished by the host blood supply 2 days after transplantation. Histologically, 3 days after grafting, metastasizing melanoma cells could be found near the vessels of the host membrane, penetrating the endothelial layer and entering the blood system. Growth conditions remained stable for 6 days after transplantation. Blood samples were taken from a larger CAM vessel before collecting the xenografts 5 days after grafting. Measurement of human S100 in peripheral blood was performed in a blinded manner. No negative control showed elevated levels of human S100 protein. Samples deriving from melanoma xenografts contained highly elevated levels of S100 protein in 80% of cases. The data strongly support the concept of graft-host interaction concerning adherence of tumors and extravasation of human melanoma cells. |
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Authors:
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K Kunzi-Rapp; P Kaskel; R Steiner; R U Peter; G Krähn |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society Volume: 14 ISSN: 0893-5785 ISO Abbreviation: Pigment Cell Res. Publication Date: 2001 Feb |
Date Detail:
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Created Date: 2001-03-29 Completed Date: 2001-07-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8800247 Medline TA: Pigment Cell Res Country: Denmark |
Other Details:
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Languages: eng Pagination: 9-13 Citation Subset: IM |
Affiliation:
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Institute for Laser Technologies and Metrology at the University of Ulm, Germany. karin.rapp@ilm.uni-ulm.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion Chick Embryo Humans Immunohistochemistry Melanoma / blood, blood supply, metabolism* Neoplasm Transplantation S100 Proteins / blood* Skin Neoplasms / blood, blood supply, metabolism |
| Chemical | |
Reg. No./Substance:
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0/S100 Proteins; 0/S100A1 protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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