Document Detail


Increased blood clearance rate of indium-111 oxine-labeled autologous CD4+ blood cells in untreated patients with Hodgkin's disease.
MedLine Citation:
PMID:  1974154     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Untreated patients with Hodgkin's disease (HD) have a blood T-lymphocytopenia mainly caused by a reduction of the CD4+ subset. Indirect support for a sequestration of T cells in the spleen and tumor-involved lymphoid tissue has accumulated. To test the hypothesis that the blood CD4 T-lymphocytopenia in patients with HD is caused by an altered lymphocyte traffic, 12 untreated HD patients and five in complete clinical remission (CCR) were studied. Blood lymphocytes were collected by leukapheresis and gradient centrifugation, and were further purified by an adherence step. The cells were labeled with indium-111 oxine and reinfused intravenously into the patient. The radioactivity of CD4+ and CD8+ blood lymphocytes separated by immunoabsorption was measured from serial blood samples. CD4+ cells were eliminated more rapidly in untreated patients than patients in CCR. Repeated gamma camera imaging after autotransfusion of indium-111 oxine labeled cells demonstrated an accumulation of radioactivity in tumor-involved tissue of untreated patients. These findings support the concept of an enhanced elimination of CD4+ cells in patients with active HD that may contribute to the observed blood T-lymphocytopenia and may reflect a biologic response to the tumor.
Authors:
G Grimfors; G Holm; H Mellstedt; P O Schnell; O Tullgren; M Björkholm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Blood     Volume:  76     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-09-11     Completed Date:  1990-09-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  583-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine, Karolinska Hospital, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antigens, CD8
Antigens, Differentiation, T-Lymphocyte / immunology
CD4-Positive T-Lymphocytes / metabolism
Female
Hodgkin Disease / immunology,  pathology*,  physiopathology
Humans
Hydroxyquinolines / diagnostic use*
Indium Radioisotopes / diagnostic use
Lymphocyte Depletion*
Male
Metabolic Clearance Rate / physiology
Middle Aged
Organometallic Compounds / diagnostic use*,  pharmacokinetics
Oxyquinoline / analogs & derivatives,  diagnostic use*,  pharmacokinetics
T-Lymphocytes / immunology,  pathology*,  physiology
T-Lymphocytes, Helper-Inducer / immunology,  pathology*,  physiology
T-Lymphocytes, Regulatory / immunology,  pathology,  physiology
Chemical
Reg. No./Substance:
0/Antigens, CD8; 0/Antigens, Differentiation, T-Lymphocyte; 0/Hydroxyquinolines; 0/Indium Radioisotopes; 0/Organometallic Compounds; 14514-42-2/indium oxine; 148-24-3/Oxyquinoline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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