| Increased arterial expression of a glycosylated haptoglobin isoform after balloon dilation. | |
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MedLine Citation:
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PMID: 12798443 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Haptoglobin is a novel cell migration factor that is expressed in arteries after sustained flow changes and involved in arterial restructuring. Arterial restructuring is the major determinant of arterial shrinkage after balloon dilation. Although the function of extrahepatic haptoglobin expression is not yet understood, local haptoglobin expression may provide the tissue with functionally different haptoglobin due to post-translational modifications. We hypothesized that haptoglobin expression is increased during arterial restructuring after balloon dilation and compared glycosylation patterns between arterial and liver haptoglobin. METHODS: Arterial haptoglobin expression was studied in rabbits at 0, 2, 7, 14 and 28 days after balloon dilation (n=36) using real-time polymerase chain reaction, Western blotting and in situ hybridization. Two-dimensional gel electrophoresis and lectin affinity blotting were used to identify liver and arterial haptoglobin glycoforms. RESULTS: Arterial haptoglobin mRNA (5.7-fold, P=0.01) and protein levels (1.4-fold, P=0.01) were increased after balloon dilation whereas liver haptoglobin expression remained constant. Haptoglobin was expressed in the adventitia of balloon dilated rabbit arteries, which was confirmed in human atherosclerotic arteries. Comparison between liver and arterial haptoglobin demonstrated the expression of artery-specific haptoglobin glycoforms. CONCLUSIONS: This study demonstrates that arterial haptoglobin expression is increased early after balloon dilation whereas liver haptoglobin expression does not change. Furthermore, arterial haptoglobin consists of an unique set of glycoforms compared to haptoglobin produced in the liver. |
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Authors:
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Mirjam B Smeets; Joost P G Sluijter; Marjo M P C Donners; Evelyn Velema; Sylvia Heeneman; Gerard Pasterkamp; Dominique P V de Kleijn |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cardiovascular research Volume: 58 ISSN: 0008-6363 ISO Abbreviation: Cardiovasc. Res. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-06-11 Completed Date: 2003-12-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 689-95 Citation Subset: IM |
Affiliation:
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Experimental Cardiology Laboratory, University Medical Center, Utrecht, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Balloon Dilatation / adverse effects* Blotting, Western / methods Coronary Restenosis / metabolism* Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Glycosylation Haptoglobins / analysis* In Situ Hybridization / methods Liver / metabolism Muscle, Smooth, Vascular / metabolism* Protein Isoforms / analysis* Rabbits Reverse Transcriptase Polymerase Chain Reaction Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Haptoglobins; 0/Protein Isoforms |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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