Document Detail

Increased apoptosis of bone marrow pre-B cells in old mice associated with their low number.
MedLine Citation:
PMID:  9786438     Owner:  NLM     Status:  MEDLINE    
The number of bone marrow pre-B cells is significantly lower in 18- than in 2-month-old BALB/c mice. The percentage of apoptotic pre-B cells, freshly isolated or cultured, from 18-month-old mice was significantly greater than from 2-month-old mice. The increased percentage of apoptotic pre-B cells from old mice was associated with a decreased level of bcl-xL mRNA, detected by RT-PCR, and of Bcl-xL protein, detected by intracellular staining. Consistent with an age-associated increase in apoptosis in pre-B cells was the fact that significantly fewer pre-B cells were generated after in vitro cultures of pro-B cells from old as compared to young mice. Furthermore, fewer pre-B cells survived and fewer sIg-expressing B cells were generated in cultures of pre-B cells from old as compared to young mice. In addition, there was no detectable difference in the secretion of IL-7 by bone marrow cells from 2- or 18-month-old mice. Thus, increased apoptosis of bone marrow pre-B cells in old mice appears to contribute to their decreased number.
I Kirman; K Zhao; Y Wang; P Szabo; W Telford; M E Weksler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International immunology     Volume:  10     ISSN:  0953-8178     ISO Abbreviation:  Int. Immunol.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-30     Completed Date:  1998-12-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8916182     Medline TA:  Int Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1385-92     Citation Subset:  IM    
Division of Geriatrics and Gerontology, Cornell University Medical College, New York, NY 10021, USA.
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MeSH Terms
Aging / physiology*
Apoptosis / physiology*
B-Lymphocytes / cytology*,  metabolism
Bone Marrow Cells / cytology*,  metabolism,  secretion
Interleukin-7 / biosynthesis,  secretion
Lymphocyte Count
Mice, Inbred BALB C
Proto-Oncogene Proteins c-bcl-2 / biosynthesis,  metabolism
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
bcl-X Protein
Grant Support
Reg. No./Substance:
0/Bcl2l1 protein, mouse; 0/Interleukin-7; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/bcl-X Protein

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