Document Detail


Increased synthetic phenotype behavior of smooth muscle cells in response to in vitro balloon angioplasty injury model.
MedLine Citation:
PMID:  19781909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Restenosis remains a common problem following balloon angioplasty, and it has been speculated that changes in the mechanical environment due to endovascular interventions are correlated with shifts in smooth muscle cell (SMC) phenotype. In order to study SMC response to forces similar to those exerted during balloon angioplasty, an in vitro concurrent shear and tensile forces simulator has been developed. After 24 hr of exposure to cyclic tension (5%) and shear (0.1-0.5 dynes/cm(2)) following simulated angioplasty injury (12% stretch), rat aortic SMCs exhibited significant synthetic behavior. These responses included increased cell proliferation, apoptosis, and cell hypertrophy compared to cells exposed to strain alone. While all SMCs exposed to dynamic stimuli (strain, strain+balloon injury, strain+balloon injury+shear) demonstrated a decrease in contractile protein expression, the injury group also exhibited significantly greater expression of the synthetic marker vimentin. These in vitro findings agree with in vivo events following balloon angioplasty and present a refined dynamic model to be implemented for better understanding of SMC activation and prevention of responses through pharmacological treatment.
Authors:
K Bethany Acampora; Jiro Nagatomi; Eugene M Langan; Martine LaBerge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-24
Journal Detail:
Title:  Annals of vascular surgery     Volume:  24     ISSN:  1615-5947     ISO Abbreviation:  Ann Vasc Surg     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703941     Medline TA:  Ann Vasc Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  116-26     Citation Subset:  IM    
Copyright Information:
Copyright 2010. Published by Elsevier Inc.
Affiliation:
Department of Bioengineering, Clemson University, 301 Rhodes Hall, Clemson, SC 29634, USA.
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MeSH Terms
Descriptor/Qualifier:
Angioplasty, Balloon / adverse effects*
Animals
Apoptosis*
Biological Markers / metabolism
Cell Proliferation*
Cell Size
Cells, Cultured
Constriction, Pathologic
Hyperplasia
In Situ Nick-End Labeling
Male
Muscle Proteins / metabolism
Muscle, Smooth, Vascular / injuries,  metabolism,  pathology*
Myocytes, Smooth Muscle / metabolism,  pathology*
Phenotype
Rats
Rats, Sprague-Dawley
Stress, Mechanical
Vimentin / metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Muscle Proteins; 0/Vimentin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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