Document Detail


Increased sperm DNA fragmentation in patients with vasectomy reversal has no prognostic value for pregnancy rate.
MedLine Citation:
PMID:  20018311     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: We evaluated sperm DNA fragmentation in patients with vasectomy reversal and its prognostic value to determine spontaneous and assisted reproductive technique pregnancy rates. MATERIALS AND METHODS: We prospectively assessed DNA fragmentation with the sperm chromatin structure assay in postoperative semen samples of 70 patients with vasectomy reversal. At a median +/- SD followup of 4.3 +/- 0.5 years pregnancy rates were recorded. RESULTS: DNA fragmentation in patients with vasectomy reversal was significantly increased vs that in proven fertile controls (30.2% +/- 20.1% vs 15.3% +/- 5.4%, p <0.001). Significant negative correlations were found between DNA fragmentation index and total sperm count, progressive motility, total number of progressive sperm, normal morphology and sperm vitality (-0.325 <r <-0.805). The obstructive interval did not correlate with DNA fragmentation. The spontaneous pregnancy rate was 46%. Significantly higher log total progressive sperm motility (p = 0.021) and a trend toward lower female age (p = 0.064) were detected in the spontaneous vs the no pregnancy group. No association was found between DNA fragmentation and the pregnancy rate. CONCLUSIONS: Increased DNA fragmentation is present in semen samples of men after vasectomy reversal vs fertile controls but DNA fragmentation is not associated with spontaneous or assisted reproductive technique pregnancy rates in these patients.
Authors:
Marij Smit; Odette G Wissenburg; Johannes C Romijn; Gert R Dohle
Publication Detail:
Type:  Journal Article     Date:  2009-12-16
Journal Detail:
Title:  The Journal of urology     Volume:  183     ISSN:  1527-3792     ISO Abbreviation:  J. Urol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-28     Completed Date:  2010-02-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  662-5     Citation Subset:  AIM; IM    
Copyright Information:
Copyright 2010 American Urological Association. Published by Elsevier Inc. All rights reserved.
Affiliation:
Andrology Unit, Department of Urology, Erasmus Medical Center Rotterdam, The Netherlands. m.smit.3@erasmusmc.nl
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MeSH Terms
Descriptor/Qualifier:
Adult
DNA Fragmentation*
Female
Humans
Male
Pregnancy / statistics & numerical data*
Prognosis
Prospective Studies
Spermatozoa*
Vasovasostomy*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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