Document Detail

Increased nitrosoglutathione reductase activity in hypoxic pulmonary hypertension in mice.
MedLine Citation:
PMID:  20431245     Owner:  NLM     Status:  MEDLINE    
Altered S-nitrosothiols (RSNO) signaling is linked to pulmonary hypertension. Recent studies have shown that S-nitrosoglutathione (GSNO) reductase (GSNOR) catalyzes the degradation of GSNO and indirectly regulates the level of RSNO in vivo. Our present study tested the hypothesis that chronic hypoxia causes pulmonary hypertension, in part, by the change of GSNOR activity that contributes to the depletion of RSNO. Male mice were exposed to normobaric hypoxia in a ventilated chamber for 1 to 21 days or normoxia for 21 days. Right ventricular systolic pressure, right ventricle hypertrophy, and the number and media thickness of muscular pulmonary vessels increased significantly after 21 days of hypoxic exposure. Hypoxia induced the overexpression of endothelial nitric oxide synthase and inducible nitric oxide synthase. The mRNA expression of GSNOR decreased on day 1 of hypoxic exposure, but increased significantly on day 7 compared with the normoxic group. The protein expression of GSNOR increased significantly in the lung tissue after 7 days of hypoxic exposure and its enzymatic activities also increased. Both the ratios of glutathione to glutathione disulfide and nitrate to nitrite were significantly lower in the hypoxic groups than in the normoxic controls. The results suggest an increased GSNOR activity interfered with the metabolism of RSNO in mice with hypoxic pulmonary hypertension. An imbalanced of redox status is associated with the pathogenesis of hypoxic pulmonary hypertension.
Xiling Wu; Lizhong Du; Xuefeng Xu; Linhua Tan; Ruyi Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-22
Journal Detail:
Title:  Journal of pharmacological sciences     Volume:  113     ISSN:  1347-8648     ISO Abbreviation:  J. Pharmacol. Sci.     Publication Date:  2010  
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-09-13     Revised Date:  2011-06-20    
Medline Journal Info:
Nlm Unique ID:  101167001     Medline TA:  J Pharmacol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  32-40     Citation Subset:  IM    
The Children's Hospital of Zhejiang University School of Medicine, China.
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MeSH Terms
Aldehyde Oxidoreductases / metabolism*
Anoxia / complications,  enzymology*,  pathology,  physiopathology
Disease Models, Animal
Gene Expression Regulation, Enzymologic
Glutathione / metabolism
Glutathione Disulfide / metabolism
Hemodynamics / physiology
Hypertension, Pulmonary / enzymology,  etiology*
Lung / metabolism,  pathology
Mice, Inbred C57BL
Models, Biological
Nitric Oxide Synthase Type II / metabolism
Nitric Oxide Synthase Type III / metabolism
Reactive Nitrogen Species / metabolism
S-Nitrosothiols / metabolism
Reg. No./Substance:
0/Reactive Nitrogen Species; 0/S-Nitrosothiols; 27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione; EC Oxide Synthase Type II; EC Oxide Synthase Type III; EC 1.2.-/Aldehyde Oxidoreductases; EC dehydrogenase, glutathione-independent

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