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Increased Mitochondrial Activity in BMP7-treated Brown Adipocytes, Due to Increased CPT1- and CD36-mediated Fatty Acid Uptake.
MedLine Citation:
PMID:  22938691     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims Brown adipose tissue dissipates chemical energy in the form of heat and regulates triglyceride and glucose metabolism in the body. Factors that regulate fatty acid uptake and oxidation in brown adipocytes have not yet been fully elucidated. Bone morphogenetic protein 7 (BMP7) is a growth factor capable of inducing brown fat mitochondrial biogenesis during differentiation from adipocyte progenitors. Administration of BMP7 to mice also results in increased energy expenditure. To determine if BMP7 is able to affect the mitochondrial activity of mature brown adipocytes, independent of the differentiation process, we delivered BMP7 to mature brown adipocytes and measured mitochondrial activity. Results We found that BMP7 increased mitochondrial activity, including fatty acid oxidation and citrate synthase activity, without increasing mitochondrial number. This was accompanied by an increase in fatty acid uptake and increased protein expression of CPT1 and CD36, which import fatty acids into the mitochondria and the cell, respectively. Importantly, inhibition of either CPT1 or CD36 resulted in a blunting of the mitochondrial activity of BMP7-treated cells. Innovation These findings uncover a novel pathway regulating mitochondrial activities in mature brown adipocytes by BMP7-mediated fatty acid uptake and oxidation. . Conclusion In conclusion, BMP7 increases mitochondrial activity in mature brown adipocytes via increased fatty acid uptake and oxidation, a process which requires the fatty acid transporters CPT1 and CD36.
Authors:
Kristy L Townsend; Ding An; Matthew D Lynes; Tian Lian Huang; Hongbin Zhang; Laurie J Goodyear; Yu-Hua Tseng
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-3
Journal Detail:
Title:  Antioxidants & redox signaling     Volume:  -     ISSN:  1557-7716     ISO Abbreviation:  Antioxid. Redox Signal.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888899     Medline TA:  Antioxid Redox Signal     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Boston, United States; kristy.townsend@joslin.harvard.edu.
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