| Increased Macroautophagy in the Pathological Process of Intervertebral Disc Degeneration in Rats. | |
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MedLine Citation:
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PMID: 22835012 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Objective: Macroautophagy increases with age in rat intervertebral discs; however, the effect of macroautophagy on the process of intervertebral disc degeneration is still unclear. The aim of this study was to examine the autophagosome presence, as well as Beclin-1 and LC3 level, in vivo. Additionally, in vitro evidence of macroautophagy and GRP78 and GADD153 level were investigated to explore the mechanism of the macroautophagy in the process of intervertebral disc degeneration. Methods: Male Sprague-Dawley rats, aged 2 months, were randomly divided into six groups (three control and three model groups, n=8 per group). At the 6-, 12-, and 18-week time points, autophagosomes in nucleus pulposus cells were detected with transmission electron microscope (TEM). Beclin-1 and LC3 expression level within intervertebral disc were detected with Western blotting. Then, the rat annulus fibrosus cells were isolated and cultured with Earle's balanced salts solution. At 1, 2 and 3 h of culture, autophagosomes were detected using monodansylcadaverine assay, and LC3, Beclin-1, GRP78 and GADD153 protein levels were detected using Western blotting. Results: TEM revealed autophagosomes within nucleus pulposus cells in both the control and model groups. At 6, 12 and 18 weeks post-treatment, the levels of Beclin-1 and the LC3-II/LC3-I protein ratio in model groups were higher than those in the control groups (p<0.05). Compared with the control rats, amino acid starvation increased the number of monodansylcadaverine-positive cells and the LC3-II/LC3-I protein ratio in the model rats. Moreover, the in vitro levels of Beclin-1, GRP78, and GADD153 proteins were increased with the prolongation of amino acid starvation (p<0.05). Conclusions: Macroutophagy was present and was associated with increased pathological process of rat intervertebral disc degeneration in rats. Macroutophagy of intervertebral disc cells is possibly secondary to endoplasmic reticulum stress. |
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Authors:
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Wei Ye; Weirong Zhu; Kang Xu; Anjing Liang; Yan Peng; Dongsheng Huang; Chunhai Li |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-26 |
Journal Detail:
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Title: Connective tissue research Volume: - ISSN: 1607-8438 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0365263 Medline TA: Connect Tissue Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1. Department of Spine Surgery, Sun Yat-sen Memorial Hospital of Sun Yat-sen University , Guangzhou , P.R China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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