| Increased glucose uptake in visceral versus subcutaneous adipose tissue revealed by PET imaging. | |
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MedLine Citation:
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PMID: 20705265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: The current study tested the hypothesis that glucose utilization differs between visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT), and investigated potential mechanisms for such a finding. BACKGROUND: VAT burden correlates better with cardiovascular risk than does SAT burden. Beyond volumetric measurement, glucose uptake in adipose tissue (AT) might reflect metabolic activity and provide pathophysiologic insight and aid risk stratification. METHODS: We retrospectively studied tissue-specific glucose uptake in vivo in clinically obtained whole-body fluorodeoxyglucose positron emission tomography (FDG-PET) scans in humans. We also assessed glucose uptake in vitro, using stromal vascular cells isolated from SAT and VAT of diet-induced obese C57BL/6 mice. Quantitative polymerase chain reaction (PCR) evaluated the expression of multiple genes involved in cellular glucose metabolism, including glucose transporters (GLUT-1, -3, and -4) and hexokinases (HK-1 and -2) in SAT and VAT of obese C57BL/6 mice. RESULTS: We analyzed whole-body FDG-PET scans from 31 obese and 26 lean patients. VAT exhibited higher FDG uptake compared with SAT (p < 0.0001) independent of age, sex, body mass index, comorbidities, and medications. To investigate mechanisms underlying this observation, we studied glucose uptake in the stromal vascular cell fraction of AT, which is rich in inflammatory cells. Stromal vascular cells from VAT of diet-induced obese C57BL/6 mice exhibited higher glucose uptake than those from SAT (p = 0.01). Evaluation of expression of glucose transporters (GLUT-1, -3, and -4) and hexokinases (HK-1 and -2), revealed increased expression of HK-1 in VAT-derived compared with SAT-derived stromal vascular cells, and also in visceral versus subcutaneous unfractionated AT. CONCLUSIONS: In humans in vivo, VAT has increased glucose uptake compared with SAT, as determined noninvasively with FDG PET imaging. Differential stromal metabolic activity may be 1 mechanism underlying differences in metabolic activity of visceral and subcutaneous AT. |
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Authors:
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Thomas Christen; Yuri Sheikine; Viviane Z Rocha; Shelley Hurwitz; Allison B Goldfine; Marcelo Di Carli; Peter Libby |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: JACC. Cardiovascular imaging Volume: 3 ISSN: 1876-7591 ISO Abbreviation: JACC Cardiovasc Imaging Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-13 Completed Date: 2010-12-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101467978 Medline TA: JACC Cardiovasc Imaging Country: United States |
Other Details:
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Languages: eng Pagination: 843-51 Citation Subset: IM |
Affiliation:
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Division of Cardiovascular Medicine, Brigham and Women's Hospital, Boston, MA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Animals Biological Transport Cells, Cultured Chi-Square Distribution Deoxyglucose / metabolism Female Fluorodeoxyglucose F18 / diagnostic use Glucose / metabolism* Glucose Transport Proteins, Facilitative / genetics, metabolism Hexokinase / genetics, metabolism Humans Intra-Abdominal Fat / metabolism*, radionuclide imaging* Kinetics Male Mice Mice, Inbred BALB C Middle Aged Obesity / metabolism*, radionuclide imaging* Positron-Emission Tomography* RNA, Messenger / metabolism Radiopharmaceuticals / diagnostic use Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction Stromal Cells / metabolism Subcutaneous Fat / metabolism*, radionuclide imaging* Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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U01HL080731/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glucose Transport Proteins, Facilitative; 0/RNA, Messenger; 0/Radiopharmaceuticals; 154-17-6/Deoxyglucose; 50-99-7/Glucose; 63503-12-8/Fluorodeoxyglucose F18; EC 2.7.1.1/Hexokinase |
| Comments/Corrections | |
Comment In:
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JACC Cardiovasc Imaging. 2010 Aug;3(8):852-3
[PMID:
20705266
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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