Document Detail

Increased frequency of micronuclei in diabetes mellitus patients using pioglitazone and glimepiride in combination.
MedLine Citation:
PMID:  20868721     Owner:  NLM     Status:  MEDLINE    
Micronuclei frequency is a sensitive biomarker used to evaluate the genotoxicity induced by xenobiotics. Pioglitazone and glimepiride were associated with genotoxicity in experimental studies conducted in rats. Considering the lack of published reports on genotoxicity in T2DM patients using pioglitazone and glimepiride drugs in combination, current study aimed to assess whether the case and control groups significantly differ from each other in the frequency of micronuclei. Subjects comprise 127 T2DM patients (35-65 years old) under pioglitazone and glimepiride treatment for >5 years and control group of 140 age matched healthy controls (38-69 years old). Exfoliated oral mucosa cells were collected from buccal mucosa of all subjects and Feulgen/Fast-Green method was followed to screen for micronuclei. Factors such as gender, food habits, living areas and occupation have not shown significant association with the variation in micronuclei frequency among the studied subjects. However, T2DM patients under long term treatment of pioglitazone and glimepiride in combination, showed increased frequency of micronuclei as compared to controls (p<0.001). Current study suggests that the micronuclei assay can be used as a constituent among the panel of biomarkers to assess genotoxicity in T2DM patients under long term antihyperglycemic drug therapy.
Noor Ahmad Shaik; Jilani Purusottapatnam Shaik; Sharique Ali; Ahamad Imran; Durga Koteswara Rao
Publication Detail:
Type:  Journal Article     Date:  2010-09-22
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  48     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-02     Completed Date:  2011-02-14     Revised Date:  2011-09-13    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3432-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Department of Genetics, Osmania University, Hyderabad, India.
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MeSH Terms
DNA Damage
Diabetes Mellitus, Type 2 / drug therapy,  pathology*
Drug Therapy, Combination
Epithelial Cells / drug effects,  ultrastructure
Genetic Markers
Hypoglycemic Agents / adverse effects*,  therapeutic use
Micronucleus Tests*
Middle Aged
Sulfonylurea Compounds / adverse effects*,  therapeutic use
Thiazolidinediones / adverse effects*,  therapeutic use
Reg. No./Substance:
0/Genetic Markers; 0/Hypoglycemic Agents; 0/Sulfonylurea Compounds; 0/Thiazolidinediones; 111025-46-8/pioglitazone; 93479-97-1/glimepiride
Comment In:
Food Chem Toxicol. 2011 Sep;49(9):2470   [PMID:  21683753 ]

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