| Increased expression of toll-like receptor 2 in rat diabetic nephropathy. | |
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MedLine Citation:
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PMID: 20628242 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: Inflammation is implicated in the pathogenesis of diabetic nephropathy (DN). This study examined the role of Toll-like receptor 2 (TLR2) in the progression of renal injury in a model of rat DN. METHODS: DN was induced by intravenous injection of streptozotocin and rats were sacrificed at week 2, 4 and 8. Functional and pathologic markers, inflammatory infiltration, expression of TLR2, MCP-1, MyD88, HSP70, HMGB1 and activation of NF-kappaB were assessed. The effects of glucose on the expression of TLR2 by renal tubular epithelial cells were also examined in vitro. RESULTS: The expression of TLR2 mRNA and protein level was significantly upregulated in the kidneys of diabetic rats (p < 0.01), which was associated with increased renal expression of MyD88 and MCP-1, activation of NF-kappaB and infiltration of macrophages. The expression of HSP70 and HMGB1, endogenous ligands of TLRs, was also significantly upregulated in the kidneys of diabetic rats. In human renal biopsy of DN, there was prominent expression of TLR2 in both the glomeruli and tubulointerstitium. In vitro study showed that high glucose induced the expression of TLR2 mRNA by NRK-52E cells (p < 0.01). CONCLUSIONS: Enhanced renal expression of TLR2 is associated with inflammatory infiltration in DN. |
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Authors:
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Fanglin Li; Niansheng Yang; Lili Zhang; Hechang Tan; Bin Huang; Yingjie Liang; Miaoyan Chen; Xueqing Yu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-14 |
Journal Detail:
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Title: American journal of nephrology Volume: 32 ISSN: 1421-9670 ISO Abbreviation: Am. J. Nephrol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-08-17 Completed Date: 2010-11-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8109361 Medline TA: Am J Nephrol Country: Switzerland |
Other Details:
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Languages: eng Pagination: 179-86 Citation Subset: IM |
Copyright Information:
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2010 S. Karger AG, Basel. |
Affiliation:
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Department of Nephrology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Cell Line Chemokine CCL2 / metabolism Diabetes Mellitus, Experimental / immunology* Diabetic Nephropathies / immunology*, pathology Female Gene Expression HMGB1 Protein / metabolism HSP70 Heat-Shock Proteins / metabolism Humans Inflammation Kidney / immunology*, metabolism, pathology Macrophages / immunology Male Middle Aged Myeloid Differentiation Factor 88 / metabolism NF-kappa B / metabolism Rats Rats, Sprague-Dawley Streptozocin Toll-Like Receptor 2 / biosynthesis*, metabolism Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Ccl2 protein, rat; 0/Chemokine CCL2; 0/HMGB1 Protein; 0/HSP70 Heat-Shock Proteins; 0/Hbp1 protein, rat; 0/Myd88 protein, rat; 0/Myeloid Differentiation Factor 88; 0/NF-kappa B; 0/TLR2 protein, human; 0/Tlr2 protein, rat; 0/Toll-Like Receptor 2; 18883-66-4/Streptozocin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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