Document Detail


Increased Expression of EphA7 in Inflamed Human Dental Pulp.
MedLine Citation:
PMID:  23321235     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
INTRODUCTION: Pulpitis has been associated with abundant inflammatory cells, dilated blood vessels, and thickening nerve fibers histopathologically with or without severe pain clinically. On the basis of EphA7 receptor expression in inflammatory cells, the developing mouse dental pulp, and trigeminal nerve system, EphA7 may possibly be involved in local inflammatory response and sensory innervation of adult dental pulp as well as odontogenic pain conducted through the trigeminal system. The purpose of the study was to analyze the expression of EphA7 gene in healthy and inflamed human dental pulps and to elucidate the roles of EphA7 gene in dental pulp inflammation response and odontogenic pain.
METHODS: Twelve healthy controls, 5 acute pulpitis from dental trauma, 21 symptomatic, and 20 asymptomatic irreversible pulpitis human dental pulps were involved in the study. The protein expression, subcellular localization, and mRNA level of EphA7 gene were detected by immunohistochemistry and real-time reverse transcriptase-polymerase chain reaction, respectively.
RESULTS: In healthy samples, immunohistochemical staining showed positive EphA7 expression only in vascular endothelial cells and odontoblasts with cytoplasm staining. Under inflammatory conditions, in addition to the above cells, EphA7 staining began to occur in fibroblasts, nerve fiber tissues, and inflammatory cells. Compared with healthy samples, EphA7 expressions at both mRNA and protein levels increased significantly in acute and irreversible pulpitis samples. In asymptomatic irreversible pulpitis samples, EphA7 expressions were significantly lower than those in symptomatic ones but still higher than those in healthy ones. There was no significant difference between acute and symptomatic irreversible pulpitis groups.
CONCLUSIONS: The results suggest that EphA7 gene may be a marker reflecting inflammatory activity and pain state for human dental pulp.
Authors:
Yingchun Dong; Weidong Lan; Weimei Wu; Zheng Huang; Junjie Zhao; Libo Peng; Jiandong Wang
Related Documents :
24661255 - Hypoxic condition promotes differentiation and mineralization of dental pulp cells in v...
24590525 - Human adult dental pulp cd117/c-kit-positive networks of stromal cells.
13618555 - The peripheral action of hexamethonium and of pentolinium.
23765615 - Phenotype-independent effects of retroviral transduction in human dental pulp stem cells.
20112925 - Synthesis of dental enamel-like hydroxyapatite through solution mediated solid-state co...
16772065 - The development, implementation, utilization and outcomes of a comprehensive dental pro...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of endodontics     Volume:  39     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  223-7     Citation Subset:  D    
Copyright Information:
Copyright © 2013 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Anesthesiology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, P. R. China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fluocinolone acetonide promotes the proliferation and mineralization of dental pulp cells.
Next Document:  Usefulness of Controlled Release of Growth Factors in Investigating the Early Events of Dentin-pulp ...