Document Detail


Increased BPA production modulates Epo sensitivity of circulating BFU-E in sickle cell anemia.
MedLine Citation:
PMID:  3223409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined the possibility that permanent "stress" hemopoiesis in sickle cell anemia (SS) patients leads to the modification of the behavior of circulating 14 day erythroid progenitor cells (BFU-E). In these patients we find that peripheral blood BFU-E are increased in number and have high sensitivity to erythropoietin (Epo). Maximal number of BFU-E are generated from peripheral blood of SS patients at 0.3-0.75 Epo/ml of culture compared to 1.5-2.0 U Epo/ml of culture in normals. Peripheral blood adherent cells depletion leads to the shift of Epo dose response curve, so that the Epo sensitivity of BFU-E significantly decreases. This result suggests that apparent Epo hypersensitivity reflects, in fact, an increased production of a burst promoting activity (BPA) by SS peripheral blood light density adherent (PB-LDA) cells. Experiments with conditioned medium by SS PB-LDA cells confirmed this interpretation. When peripheral blood light density non-adherent (PB-LDNA) cells of SS patients or normal individuals were plated in the presence of various concentrations of SS PB-LD cells conditioned medium and constant amounts of Epo, a dose dependent increase of the number of BFU-E was observed. When the same target cells were plated in the presence of PB-LD cells conditioned medium from normal individuals, such effect does not occur. We conclude that increased BPA production may play a role in the erythropoietic regulation during constant hemopoietic stress in sickle cell anemia and might partially explain the lower than expected Epo levels in these patients.
Authors:
H Croizat; R L Nagel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  241     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  1988  
Date Detail:
Created Date:  1989-03-17     Completed Date:  1989-03-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  311-7     Citation Subset:  IM    
Affiliation:
Albert Einstein College of Medicine, Department of Medicine, Bronx, New York.
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MeSH Terms
Descriptor/Qualifier:
Anemia, Sickle Cell / physiopathology*
Cells, Cultured
Dose-Response Relationship, Drug
Erythropoiesis*
Erythropoietin / pharmacology*
Hematopoietic Stem Cells / physiology*
Humans
Methylcellulose
Grant Support
ID/Acronym/Agency:
HL21016/HL/NHLBI NIH HHS; T32 HL07556/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
11096-26-7/Erythropoietin; 9004-67-5/Methylcellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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