Document Detail


Increased CCL24/eotaxin-2 with postnatal ozone exposure in allergen-sensitized infant monkeys is not associated with recruitment of eosinophils to airway mucosa.
MedLine Citation:
PMID:  21945493     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epidemiology supports a causal link between air pollutant exposure and childhood asthma, but the mechanisms are unknown. We have previously reported that ozone exposure can alter the anatomic distribution of CD25+ lymphocytes in airways of allergen-sensitized infant rhesus monkeys. Here, we hypothesized that ozone may also affect eosinophil trafficking to allergen-sensitized infant airways. To test this hypothesis, we measured blood, lavage, and airway mucosa eosinophils in 3-month old monkeys following cyclical ozone and house dust mite (HDM) aerosol exposures. We also determined if eotaxin family members (CCL11, CCL24, CCL26) are associated with eosinophil location in response to exposures. In lavage, eosinophil numbers increased in animals exposed to ozone and/or HDM. Ozone+HDM animals showed significantly increased CCL24 and CCL26 protein in lavage, but the concentration of CCL11, CCL24, and CCL26 was independent of eosinophil number for all exposure groups. In airway mucosa, eosinophils increased with exposure to HDM alone; comparatively, ozone and ozone+HDM resulted in reduced eosinophils. CCL26 mRNA and immunofluorescence staining increased in airway mucosa of HDM alone animals and correlated with eosinophil volume. In ozone+HDM animal groups, CCL24 mRNA and immunofluorescence increased along with CCR3 mRNA, but did not correlate with airway mucosa eosinophils. Cumulatively, our data indicate that ozone exposure results in a profile of airway eosinophil migration that is distinct from HDM mediated pathways. CCL24 was found to be induced only by combined ozone and HDM exposure, however expression was not associated with the presence of eosinophils within the airway mucosa.
Authors:
Debbie L Chou; Joan E Gerriets; Edward S Schelegle; Dallas M Hyde; Lisa A Miller
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-09-12
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  257     ISSN:  1096-0333     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-29     Completed Date:  2012-01-13     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  309-18     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Allergens / immunology
Animals
Animals, Newborn
Chemokine CCL24 / immunology,  metabolism*
Eosinophils / immunology,  metabolism*
Fluorescent Antibody Technique
Macaca mulatta
Male
Ozone / immunology,  toxicity*
Pyroglyphidae / immunology
RNA, Messenger / metabolism
Respiratory Mucosa / immunology,  metabolism*
Grant Support
ID/Acronym/Agency:
AI065567/AI/NIAID NIH HHS; ES-00628/ES/NIEHS NIH HHS; ES-007059/ES/NIEHS NIH HHS; ES-11617/ES/NIEHS NIH HHS; HL-07013/HL/NHLBI NIH HHS; HL-81286/HL/NHLBI NIH HHS; P01 ES000628/ES/NIEHS NIH HHS; P01 ES000628-36/ES/NIEHS NIH HHS; P01 ES011617-08/ES/NIEHS NIH HHS; P51 RR000169-48/RR/NCRR NIH HHS; R01 HL081286/HL/NHLBI NIH HHS; R01 HL081286-04/HL/NHLBI NIH HHS; R01 HL097087/HL/NHLBI NIH HHS; R01 HL097087-03/HL/NHLBI NIH HHS; R03 AI065567/AI/NIAID NIH HHS; R03 AI065567-02/AI/NIAID NIH HHS; RR-00169/RR/NCRR NIH HHS; T32 ES007059-32/ES/NIEHS NIH HHS; T32 HL007013-34/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Allergens; 0/Chemokine CCL24; 0/RNA, Messenger; 66H7ZZK23N/Ozone
Comments/Corrections

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