Document Detail


Increase of sialylated tetraantennary sugar chains in parallel to the higher lung-colonizing abilities of mouse melanoma clones.
MedLine Citation:
PMID:  1940447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The N-linked sugar chains of melanoma cell membrane from five murine B16 melanoma clones (F1, F10, BL6, W1-4, and C4-1) with different degrees of metastatic abilities after intravenous and intrafootpad injections were released quantitatively as oligosaccharides by hydrazinolysis, and their structures were analyzed by serial lectin column chromatography, Bio-Gel P-4 column chromatography, and sequential glycosidase digestion. Sugar chain structures of each clone have shown to consist of the same elemental oligosaccharides, but to differ in their percent compositions. More than 84% of the neutral oligosaccharides were high mannose-type sugar chains. Most complex-type sugar chains were sialylated, of which the major structure was tetraantennary sugar chain. Highly lung-colonizing F10 cells had 1.4 and 1.7 times more non-repeated tetraantennary sugar chains than moderately colonizing F1 and C4-1 cells, respectively, and 2.5 times more than poorly colonizing W1-4 cells. BL6 cells, which are also highly lung-colonizing, had 1.5 and 1.9 times more non-repeated tetraantennary sugar chains than F1 and C4-1 cells, respectively, and 2.8 times more than W1-4 cells. These results suggest that increase of sialylated tetraantennary complex-type sugar chains without N-acetyllactosamine repeating units of B16 melanoma cells might correlate with the higher lung-colonizing ability after intravenous injection.
Authors:
K Yamamura; S Takasaki; M Ichihashi; Y Mishima; A Kobata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  97     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1991-11-27     Completed Date:  1991-11-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  735-41     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Kobe University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrate Sequence
Chromatography, Affinity
Clone Cells
Lung Neoplasms / secondary*
Melanoma, Experimental / chemistry*,  pathology
Mice
Molecular Sequence Data
Oligosaccharides / analysis*,  chemistry
Sialic Acids / analysis
Chemical
Reg. No./Substance:
0/Oligosaccharides; 0/Sialic Acids

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