| Increase of oxidant-related triglycerides and phosphatidylcholines in serum and small intestinal mucosa during development of intestinal polyp formation in Min mice. | |
| | |
MedLine Citation:
|
PMID: 20946475 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Recent epidemiological studies have shown a positive association of a high-fat diet with the risk of colon cancer. Indeed, increments in the serum levels of triglycerides (TG) and cholesterols are positively related with colon carcinogenesis. We previously reported that an age-dependent hyperlipidemic state is characteristic of Min mice, an animal model for human familial adenomatous polyposis (FAP). However, qualitative and quantitative changes of lipid metabolism are poorly understood in this state. Here, we provide detailed analysis of serum lipids in Min mice using reverse-phased liquid chromatography/electrospray ionization mass spectrometry (RPLC/ESI-MS). We also demonstrate local analysis of lipid droplets in the villi of the small intestine using laser capture microdissection and a sensitive chip-based nanoESI-MS system. As a result, oxidized phosphatidylcholines (PC) such as aldehyde and carboxylic acid types were increased, even at an early stage of intestinal polyp formation in serum. In addition, hydroperoxidizable TG precursors containing linoleic acid (18:2n-6) were deposited at the tip of the villi with aging, and these hydroperoxidized TG were also increased in serum. Meanwhile, increments of the oxidizable TG precursors in serum and small intestinal mucosa were suppressed by treatment with pitavastatin, a novel third generation lipophilic statin. These results suggest that quantitative and qualitative lipid changes such as hydroperoxidizable TG precursors are important in the course of intestinal polyp formation and oxidative stress might lead to the development of intestinal polyp formation in Min mice. |
| | |
Authors:
|
Kazutaka Ikeda; Michihiro Mutoh; Naoya Teraoka; Hiroki Nakanishi; Keiji Wakabayashi; Ryo Taguchi |
Related Documents
:
|
16549465 - Baseline plasma c-reactive protein concentrations influence lipid and lipoprotein respo... 21880475 - α-linolenate reduces the dietary requirement for linoleate in the growing rat. 18380465 - Lipid-lowering and antioxidant effects of hydroxytyrosol and its triacetylated derivati... 9614165 - Alimentary lipemia is enhanced in fiber-fed rats. 9373365 - Evaluation of ruminal sulfide concentrations and seasonal outbreaks of polioencephaloma... 7955075 - Consumption of reduced-energy/low-fat diet or constant-energy/high-fat diet during meze... |
Publication Detail:
|
Type: Journal Article Date: 2010-10-14 |
Journal Detail:
|
Title: Cancer science Volume: 102 ISSN: 1349-7006 ISO Abbreviation: Cancer Sci. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-16 Completed Date: 2011-01-18 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101168776 Medline TA: Cancer Sci Country: England |
Other Details:
|
Languages: eng Pagination: 79-87 Citation Subset: IM |
Copyright Information:
|
© 2010 Japanese Cancer Association. |
Affiliation:
|
Department of Metabolome, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenomatous Polyposis Coli Protein
/
deficiency* Animals Intestinal Mucosa / metabolism Intestinal Polyps / genetics*, metabolism* Intestine, Small / metabolism* Lipid Peroxidation Male Mice Mice, Inbred C57BL Phosphatidylcholines / metabolism* Quinolines / pharmacology Spectrometry, Mass, Electrospray Ionization Triglycerides / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Adenomatous Polyposis Coli Protein; 0/Phosphatidylcholines; 0/Quinolines; 0/Triglycerides; 147511-69-1/pitavastatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Proteolytic activation of heparin-binding EGF-like growth factor by membrane-type matrix metalloprot...
Next Document: The effectiveness of self-directed learning in health professions education: a systematic review.