Document Detail

Increase in vulnerability to oxidative damage in cholesterol-modified erythrocytes exposed to t-BuOOH.
MedLine Citation:
PMID:  15866485     Owner:  NLM     Status:  MEDLINE    
During the course of radical oxidation, cholesterol may exert seemingly contradictory effects. In order to gain a better understanding of the relationship between cholesterol levels and membrane susceptibility to oxidative damage induced by reactive oxygen species (ROS), here we analyze the integrity and structural stability of cholesterol-modified (enriched or depleted) and unmodified (control) erythrocytes exposed to tert-butyl hydroperoxide. The oxidant significantly increased ROS production, with almost complete oxidation of hemoglobin and a reduction in GSH content in the different erythrocyte groups at 2 mM concentration. These changes were accompanied by losses of cholesterol and total phospholipids, the main decreases being in phosphatidylethanolamine and phosphatidylcholine. The highest lipid loss was found in the cholesterol-depleted group. Fatty acid analyses revealed changes only in peroxidized cholesterol-modified erythrocytes, with decreases in linoleic and arachidonic acids. Fluorescence anisotropy studies showed an increase in the fluidity of the negatively charged surface of peroxidized control erythrocytes. Increased hemolysis and a positive correlation between cellular osmotic fragility and malondialdehyde contents were found in all peroxidized groups. These findings provide evidence that the modification of cholesterol levels in the erythrocyte membrane has provoking effects on peroxidation, with corresponding increases in oxidative damage in the treated cell, possibly as a consequence of lipid bilayer destabilization.
Abel López-Revuelta; José I Sánchez-Gallego; Angel Hernández-Hernández; Jesús Sánchez-Yagüe; Marcial Llanillo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-03-05
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1734     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-03     Completed Date:  2005-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  74-85     Citation Subset:  IM    
Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Plaza Doctores de la Reina s/n, 37007 Salamanca, Spain.
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MeSH Terms
Cholesterol / metabolism*
Erythrocyte Membrane / metabolism*
Hemoglobins / metabolism
Hemolysis / drug effects
Lipid Peroxidation / drug effects
Malondialdehyde / metabolism
Osmotic Fragility / drug effects
Oxidation-Reduction / drug effects
Oxidative Stress / drug effects*
Phosphatidylcholines / metabolism
Phosphatidylethanolamines / metabolism
Reactive Oxygen Species / metabolism
tert-Butylhydroperoxide / pharmacology*
Reg. No./Substance:
0/Hemoglobins; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Reactive Oxygen Species; 39382-08-6/phosphatidylethanolamine; 542-78-9/Malondialdehyde; 57-88-5/Cholesterol; 75-91-2/tert-Butylhydroperoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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