| Increase in synaptic vesicle proteins in synapsin-transfected NG108-15 cells: a subcellular fractionation study. | |
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MedLine Citation:
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PMID: 7624829 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have examined the subcellular distribution of synapsins and synaptophysin in density gradients from synapsin- and vector-transfected NG108-15 cells, since we recently found that transfection of synapsin IIb cDNA into neuroblastoma x glioma hybrid cells (NG108-15) resulted in cell lines that had a more neuronal phenotype than controls. The increase in synapsins and synaptophysin in the transfected cells was maximal in the region of the gradient containing small synaptic vesicles. The transferrin receptor, a marker for early endosomes, did not increase in the synapsin-containing fractions in the transfected cells. Secretogranin I, a soluble protein stored in and secreted from large dense cored vesicles, showed a very pronounced increase in the dense regions of gradients from transfected cells. These subcellular fractionation data suggest a possible role for the synapsins in the regulation of synaptic vesicle function. |
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Authors:
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G Fried; H Q Han |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Synapse (New York, N.Y.) Volume: 20 ISSN: 0887-4476 ISO Abbreviation: Synapse Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-08-31 Completed Date: 1995-08-31 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8806914 Medline TA: Synapse Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 44-53 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Centrifugation, Density Gradient DNA / biosynthesis Electrophoresis, Polyacrylamide Gel Immunoblotting Immunohistochemistry Microscopy, Immunoelectron Nerve Tissue Proteins / metabolism* Neuroblastoma / metabolism Rats Subcellular Fractions / metabolism Synapsins / metabolism* Synaptic Vesicles / metabolism*, ultrastructure Transfection Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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MH-39327/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nerve Tissue Proteins; 0/Synapsins; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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