Document Detail


Increase in ribonuclease activity following mechanical damage to leaf and tuber tissues of Solanum tuberosum L.
MedLine Citation:
PMID:  24488475     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Major increases occurred in the capacity of damaged potato leaf and tuber tissues to hydrolyse ribonucleic acid whilst relatively minor increases were found in the activity of acid phosphomonoesterase and acid phosphodiesterase. Partial purification of homogenates by gel filtration on Sephadex G-100 revealed that much of the increased capacity to degrade ribonucleic acid following damage was due to increased ribonuclease activity. Although appreciable differences in the elution patterns of tuber homogenates subjected to gel filtration were observed before and after the breaking of dormancy the increased ribonuclease activity following damage was a constant feature. Actinomycin D had a relatively small effect on preventing these increases in phosphate-ester hydrolase activities whilst the effect of cycloheximide was very pronounced. Isopycnic equilibrium centrifugation experiments, using deuterium oxide as a density label, provided no evidence that the increased enzyme activity following damage was due to synthesis of new enzyme.
Authors:
D Pitt; M Galpin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta     Volume:  101     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  1971 Dec 
Date Detail:
Created Date:  2014-02-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  317-32     Citation Subset:  -    
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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