Document Detail

Increase in prevalence of human group A rotavirus G9 strains as an important VP7 genotype among children in eastern India.
MedLine Citation:
PMID:  18755625     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: In addition to four globally important group A rotavirus (GARV) VP7 genotypes (G1-G4), recent surveillance studies have revealed importance of G9 strains as an aetiological agent of infantile diarrhoea. OBJECTIVE: Detection and genotyping of GARVs from children, admitted with gastroenteritis to Dr. B.C. Roy Memorial Hospital for Children, Kolkata, India. STUDY DESIGN: GARVs were detected in stool specimens by RNA electrophoresis in polyacrylamide gels. G- and P-genotyping were performed by seminested multiplex PCR assays. VP7 gene of rotavirus G9 and G12 strains were sequenced for further analysis. RESULTS: Of 249 GARV strains (n=668, May 2005-December 2006), G- and P-genotyping were successfully accomplished for 197 and 204 samples, respectively. G1 (41.6%) was most prevalent G-genotype followed by G2 (33%), G12 (14.2%), G9 (10.1%) and mixed genotype (1%). Prevalent P-genotypes were P[8] (54.4%), P[4] (31.4%), P[6] (7.3%) and mixed genotype (6.9%). Overall, G1P[8], G2P[4], G9P[8], G12P[8] and G12P[6] were identified as important G-P combinations. Phylogenetic analysis of 13 G9 strains revealed clustering within G9 lineage III. Nine of 28 G12 strains were sequenced and exhibited phylogenetic clustering with previously reported G12 strains from Kolkata. CONCLUSION: In comparison to our previous data (2003 to April 2005), G9 and G2P[4] strains established themselves in a short time span as important genotypes in eastern India.
Sudipta Samajdar; Souvik Ghosh; Mamta Chawla-Sarkar; Utpala Mitra; Phalguni Dutta; Nobumichi Kobayashi; Trailokya N Naik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-28
Journal Detail:
Title:  Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology     Volume:  43     ISSN:  1386-6532     ISO Abbreviation:  J. Clin. Virol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-22     Completed Date:  2009-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815671     Medline TA:  J Clin Virol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  334-9     Citation Subset:  IM    
Division of Virology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road, Scheme XM, Beliaghata, Kolkata 700010, West Bengal, India.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ117937;  DQ117938;  DQ117939;  EF585201;  EF585203;  EF585206;  EF585207;  EF585208;  EF585209;  EF585211;  EF585212;  EF585213;  EU016451;  EU016452;  EU016453;  EU016454;  EU016455;  EU016456;  EU016457;  EU016460;  EU016464
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MeSH Terms
Antigens, Viral / genetics
Capsid Proteins / genetics
Child, Preschool
Cluster Analysis
Electrophoresis, Polyacrylamide Gel
Feces / virology
Gastroenteritis / epidemiology*,  virology*
India / epidemiology
Molecular Sequence Data
Polymerase Chain Reaction / methods
RNA, Viral / genetics,  isolation & purification
Rotavirus / classification*,  genetics,  isolation & purification*
Rotavirus Infections / epidemiology*,  virology*
Sequence Analysis, DNA
Sequence Homology
Reg. No./Substance:
0/Antigens, Viral; 0/Capsid Proteins; 0/RNA, Viral; 0/VP7 protein, Rotavirus

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