| Increase in milk metalloproteinase activity and vascular permeability in bovine endotoxin-induced and naturally occurring Escherichia coli mastitis. | |
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MedLine Citation:
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PMID: 11943315 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An endotoxin-induced mastitis model was used to study permeability changes associated with increased milk matrix metalloproteinase (MMP) activity in early inflammation. One quarter of two cows was inoculated with endotoxin (Escherichia coli 055:B5). Blood, milk, and whey were collected before and repeatedly after inoculation for 48 h. The profile and amounts of gelatinolytic MMPs were determined by zymography; gelatinase A (72 kD MMP-2) and gelatinase B (92 kD MMP-9) were identified by Western immunoblotting. Bovine serum albumin (BSA) and trypsin inhibitor capacity (TIC) were used as markers of capillary permeability with parallel examination of neutrophil penetration from blood to milk. Five clinical E. coli mastitis milk samples and five milk samples from cows with healthy udders were analyzed to detect whether increased levels of gelatinolytic MMP-2 and MMP-9 have a role in naturally occurring mastitis with endotoxin involvement. Milk MMP levels increased 2h after the endotoxin challenge. Both MMP-2 and MMP-9 were involved in this early proteolytic event. These increased MMP levels are associated with increased capillary permeability, evidenced first by the penetration of small molecular weight proteins, such as BSA and TIC. Later, 6-12h post endotoxin inoculation, neutrophilic leucocytes also entered the site, as they require larger tissue damage in basal membrane and interstitial tissue structures than BSA and TIC to extravasate. In naturally occurring disease, increased MMP-2 and MMP-9 levels were detected in milk. Thus, gelatinases, especially MMP-2, are involved in the early degradation of the blood-milk barrier, which precedes the penetration of blood-derived cellular components into milk in endotoxin-induced mastitis. In the future, measuring MMP in milk/whey might be a useful tool for diagnosing early mastitis. |
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Authors:
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S M Raulo; T Sorsa; T Tervahartiala; T Latvanen; E Pirilä; J Hirvonen; P Maisi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Veterinary immunology and immunopathology Volume: 85 ISSN: 0165-2427 ISO Abbreviation: Vet. Immunol. Immunopathol. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-04-10 Completed Date: 2002-06-12 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8002006 Medline TA: Vet Immunol Immunopathol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 137-45 Citation Subset: IM |
Affiliation:
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Faculty of Veterinary Medicine, Department of Clinical Veterinary Sciences, Helsinki University, P.O. Box 57, Hämeentie 57, Helsinki FIN-00014, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western / veterinary Capillary Permeability / immunology Cattle Electrophoresis, Polyacrylamide Gel / veterinary Endotoxins / metabolism, toxicity Escherichia coli* / metabolism Escherichia coli Infections / enzymology, immunology, veterinary* Female Leukocyte Count / veterinary Mastitis, Bovine / blood, enzymology*, immunology Matrix Metalloproteinase 2 / biosynthesis*, metabolism Matrix Metalloproteinase 9 / biosynthesis*, metabolism Milk / enzymology* Milk Proteins / analysis Plant Proteins / blood Serum Albumin, Bovine / analysis Trypsin Inhibitors alpha-Amylases / antagonists & inhibitors |
| Chemical | |
Reg. No./Substance:
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0/Endotoxins; 0/Milk Proteins; 0/Plant Proteins; 0/Serum Albumin, Bovine; 0/Trypsin Inhibitors; 0/whey protein; EC 3.2.1.1/alpha-Amylases; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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