Document Detail


Increase in interleukin-8 production from circulating neutrophils upon antibiotic therapy in cystic fibrosis patients.
MedLine Citation:
PMID:  22608703     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: It is not known whether antibiotic therapy for lung disease in cystic fibrosis (CF) has an influence on circulating polymorphonuclear neutrophil (PMN) function and apoptosis. PATIENTS AND METHODS: Blood PMNs were obtained from 14 CF patients before and after antibiotic treatment for an acute exacerbation, and from 10 healthy controls. PMNs were evaluated for production of reactive oxygen species (ROS) by spectrophotometry, of cytokines in the conditioned medium by ELISA, and apoptotic response by cytofluorimetry. RESULTS: ROS and interleukin (IL)-8 were produced at higher levels by CF PMNs pre-therapy than control PMNs under basal conditions. IL-8 levels further increased after therapy. Early apoptotic response was higher in CF PMNs pre-therapy than in control PMNs, and this pattern did not change after antibiotic treatment. CONCLUSIONS: Circulating PMNs are primed in CF acute patients. Further studies are needed to consider PMN-produced IL-8 as a biomarker to evaluate response to antibiotic therapy in CF patients.
Authors:
Pasqualina Montemurro; Maria A Mariggiò; Giovanna Barbuti; Amalia Cassano; Alessandra Vincenti; Gabriella Serio; Lorenzo Guerra; Anna Diana; Teresa Santostasi; Angela Polizzi; Ruggiero Fumarulo; Valeria Casavola; Antonio Manca; Massimo Conese
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-17
Journal Detail:
Title:  Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society     Volume:  -     ISSN:  1873-5010     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128966     Medline TA:  J Cyst Fibros     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
Affiliation:
Department of Biomedical Sciences and Human Oncology, Section of General Pathology, University of Bari, Bari, Italy.
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