Document Detail


Increase in hepatic mitochondrial alpha-glycerophosphate dehydrogenase activity after surgical stress in hyperthyroid rats.
MedLine Citation:
PMID:  2361477     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have established a rat model to investigate the relationship among serum thyroid hormones, nuclear iodothyronine receptors, and biological responses in thyrotoxic and euthyroid rats after surgical stress. Euthyroid or hyperthyroid rats (1.0 microgram T3/ml drinking water for 14 days) were subjected to surgical stress (ether anesthesia, laparotomy plus 50 mg talc, ip). Groups of control or stressed rats were killed 1, 2, and 3 days after surgical stress for measurement of thyroid hormone-responsive hepatic enzymes, alpha-glycerophosphate dehydrogenase (alpha GPD) and cytosol malic enzyme, serum T3, T3 nuclear receptors, and GH mRNA. Thyrotoxic rats had a 3.8-fold increase in alpha GPD compared to euthyroid rats before surgical stress; alpha GPD increased further to 5.9-fold the euthyroid value 1 day after surgery (P less than 0.001) to 5.1-fold after 2 days (P less than 0.05) and was similar to control after 3 days. Malic enzyme activity increased 10.5-fold before surgical stress and decreased slightly after surgical stress perhaps due to multifactorial regulation of that enzyme. No increases in T3 nuclear receptor or GH mRNA occurred after surgery in hyperthyroid rats or in any of the above parameters after surgical stress in euthyroid rats. Our findings suggest that increased alpha GPD after surgical stress in thyrotoxic rats was not due to either increased serum total T3 or free T3 or to increased T3-nuclear receptor complexes. Increased alpha GPD, therefore, appeared to be a consequence of postreceptor amplification of this thyroidal response.
Authors:
Y Khawaja; H Dobnig; L E Shapiro; M I Surks
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  127     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-08-08     Completed Date:  1990-08-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  387-93     Citation Subset:  AIM; IM    
Affiliation:
Division of Endocrinology and Metabolism, Montefiore Medical Center, Bronx, New York 10467.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / metabolism
Cytosol / enzymology
Glycerolphosphate Dehydrogenase / metabolism*
Growth Hormone / genetics
Hyperthyroidism / enzymology*
Malate Dehydrogenase / metabolism
Male
Mitochondria, Liver / enzymology*
RNA, Messenger / metabolism
Rats
Receptors, Thyroid Hormone / metabolism
Stress, Physiological / enzymology*
Surgical Procedures, Operative*
Triiodothyronine / administration & dosage,  blood
Grant Support
ID/Acronym/Agency:
CA-16463-15/CA/NCI NIH HHS; CA-24604-10/CA/NCI NIH HHS; DK-07004-16/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Thyroid Hormone; 6893-02-3/Triiodothyronine; 9002-72-6/Growth Hormone; EC 1.1.-/Glycerolphosphate Dehydrogenase; EC 1.1.1.37/Malate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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