Document Detail

Increase in fatty acid synthetase content of 3T3-L cells undergoing spontaneous and chemically induced differentiation to adipocytes.
MedLine Citation:
PMID:  92315     Owner:  NLM     Status:  MEDLINE    
3T3-L fibroblasts differentiate into adipose cells when maintained in a non-growing state. The specific activity of fatty acid synthetase of differentiated cells was 25--30-fold higher than that present in 3T3-L fibroblasts or in 3T3-C2 cells that possess an extremely low incidence of differentiation to adipocytes. The results of immunochemical analysis indicate that the increased specific activity of fatty acid synthetase in the differentiated cells is due to an increase in the cellular content of this enzyme. The rate of conversion of adipose cells was accelerated by brief exposure of confluent non-growing cultures of 3T3-L cells to 3-isobutyl-1-methylxanthine. This was accompanied by an increase in the specificity activity of fatty acid synthetase, which was also shown to be due to an increase in the cellular content of this enzyme. The continuous presence of 3-isobutyl-1-methylxanthine in the culture medium was not required to elicit the morphological and biochemical changes in 3T3-L cells that occurred many days after the removal of the inducer but earlier than the onset of spontanous differentiation.
P M Ahmad; T R Russell; F Ahmad
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  182     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1979 Aug 
Date Detail:
Created Date:  1980-01-24     Completed Date:  1980-01-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  509-14     Citation Subset:  IM    
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MeSH Terms
1-Methyl-3-isobutylxanthine / pharmacology
Adipose Tissue / cytology*,  enzymology
Cell Differentiation
Cell Line
Fatty Acid Synthetase Complex / immunology,  metabolism*
Fibroblasts / cytology,  drug effects,  enzymology*
Reg. No./Substance:
28822-58-4/1-Methyl-3-isobutylxanthine; EC 6.-/Fatty Acid Synthetase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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