Document Detail


Increase in 15-hydroxyprostaglandin dehydrogenase activity in the ovine placentome at parturition and effect of oestrogen.
MedLine Citation:
PMID:  10864846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Type 1 NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) is the key enzyme for metabolism of active primary prostaglandins to inactive forms in gestational tissues. The present study examined the activity and immunolocalization of PGDH in the ovine placenta, fetal membranes and uterus over the latter half of pregnancy, and its potential regulation by oestradiol. Placenta, fetal membranes and myometrium were collected from sheep with known single insemination dates on days 70, 100 and 135 of gestation and in active labour demonstrated by electromyographic activity. In addition, chronically catheterized fetuses were infused with oestradiol (100 microgram kg(-1) per 24 h) (n = 5) or saline vehicle into the fetus from day 120 to day 125. PGDH activity measured in placental extracts remained constant from day 70 to day 135 of gestation, and then significantly (P < 0.05) increased by 300% in active labour. Immunoreactive PGDH was localized in the placentome at all stages and was present predominantly in the fetal component of the placentome in uninucleate, but not in binucleate, trophoblast cells. Similarly, in the fetal membranes PGDH immuno-reactivity was present in the uninucleate trophoblast but not in the binucleate cells of the chorion. PGDH immunostaining was also present in the endometrial luminal epithelium, in the smooth muscle of the myometrium, and the glandular epithelium of the cervix. Infusion of oestradiol into the fetal circulation from day 120 to day 125 of gestation had no effect on placental PGDH activity. Immunohistochemistry was used to localize oestrogen receptor alpha in intrauterine tissues to investigate further the failure of oestradiol to increase PGDH activity. Immunoreactive oestrogen receptor alpha was not present in the fetal component of the placenta, although it was expressed in adjacent maternal-derived cells. It is concluded that (1) PGDH activity increases in late gestation; (2) PGDH is expressed in uninucleate trophoblast cells in the ovine placenta and fetal membranes, and also in the maternal endometrial epithelium and stroma, myometrium and cervix; (3) oestrogen receptor alpha is not expressed in fetal cells in the placenta or fetal membranes; and (4) the increase in PGDH activity is not regulated by oestradiol administered to the fetus.
Authors:
S C Riley; R Leask; J V Selkirk; R W Kelly; A N Brooks; D C Howe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  119     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-09-12     Completed Date:  2000-09-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  329-38     Citation Subset:  IM    
Affiliation:
Department of Reproductive and Developmental Sciences, Obstetrics and Gynaecology Section, University of Edinburgh, Edinburgh, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cervix Uteri / enzymology
Endometrium / enzymology
Estradiol / pharmacology
Female
Gestational Age
Hydroxyprostaglandin Dehydrogenases / metabolism*
Immunohistochemistry
Labor, Obstetric / metabolism*
Myometrium / enzymology
Placenta / enzymology*
Pregnancy
Prostaglandins / metabolism*
Receptors, Estrogen / analysis
Sheep / metabolism*
Trophoblasts / drug effects,  enzymology
Chemical
Reg. No./Substance:
0/Prostaglandins; 0/Receptors, Estrogen; 50-28-2/Estradiol; EC 1.1.1.-/Hydroxyprostaglandin Dehydrogenases; EC 1.1.1.141/15-hydroxyprostaglandin dehydrogenase

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