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Increase of NADPH-diaphorase expression in hypothalamus of stat4 knockout mice.
MedLine Citation:
PMID:  19915694     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Signal transducer and activator of transcription 4 (STAT4), a STAT family member, mediates interleukin 12 (IL12) signal transduction. IL12 is known to be related to calorie-restricted status. In the central nervous system, IL12 also enhances the production of nitric oxide (NO), which regulates food intake. In this study, the expression of neuronal NO synthase (Nos1), which is also related to food intake, was investigated in the hypothalamic areas of Stat4 knockout (KO) mice using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry, a marker for neurons expressing Nos1 enzyme. Western blots were also performed to evaluate Nos1 and Fos expression. Wild-type Balb/c (WT group, n=10 male) and Stat4 KO mice (Stat4 KO group, n=8 male) were used. The body weight and daily food intake in the WT group were 22.4+/-0.3 and 4.4 g per day, while those in the Stat4 KO group were 18.7+/-0.4 and 1.8 g per day, respectively. Stat4 mice had lower body weight and food intake than Balb/c mice. Optical intensities of NADPH-d-positive neurons in the paraventricular nucleus (PVN) and lateral hypothalamic area (LHA) of the Stat4 KO group were significantly higher than those of the WT group. Western blotting analysis revealed that the hypothalamic Nos1 and Fos expression of the Stat4 KO group was up-regulated, compared to that in the WT group. These results suggest that Stat4 may be related to the regulation of food intake and expression of Nos1 in the hypothalamus.
Mee Sook Hong; Jeong Yoon Song; Dong Hwan Yun; Jeong-Je Cho; Joo-Ho Chung
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Publication Detail:
Type:  Journal Article     Date:  2009-10-31
Journal Detail:
Title:  The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology     Volume:  13     ISSN:  1226-4512     ISO Abbreviation:  Korean J. Physiol. Pharmacol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-11-16     Completed Date:  2011-07-14     Revised Date:  2013-05-23    
Medline Journal Info:
Nlm Unique ID:  9709505     Medline TA:  Korean J Physiol Pharmacol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  337-41     Citation Subset:  -    
Kohwang Medical Research Institute, Kyung Hee University, Seoul 130-701, Korea.
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