| Increase of 27-hydroxycholesterol in the airways of patients with chronic obstructive pulmonary disease: possible role of 27-hydroxycholesterol in tissue fibrosis. | |
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MedLine Citation:
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PMID: 22281802 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract BACKGROUND:27-Hydroxycholesterol (27-OHC) is produced from cholesterol by sterol 27-hydroxylase as an intermediate in the biosynthesis pathway of bile acid. Recently, 27-OHC was reported to cause inflammation and apoptosis in various types of cells. The aim of this study is to assess the production of 27-OHC in the airways of chronic obstructive pulmonary disease (COPD) and to elucidate the possible role of 27-OHC in the tissue fibrosis of COPD. METHODS:Lung tissues were obtained from 6 control subjects and 6 COPD patients and sputum samples were obtained from 11 healthy subjects and 15 COPD patients. The expression of sterol 27-hydroxylase in the lung was investigated by immunohistochemistry. The amounts of 27-OHC in the sputum were quantified by liquid chromatography-tandem mass spectrometry method. Because peribronchial fibrosis in peripheral airways is involved in the airflow limitation of COPD, we investigated the pro-fibrotic effects of 27-OHC in vitro. RESULTS:The expression of sterol 27-hydroxylase was significantly enhanced in the lung tissues from COPD patients compared to control subjects. The amounts of 27-OHC in the sputum were significantly increased in COPD patients (p < 0.01) and the degree of 27-OHC production was negatively correlated with the lung function (p < 0.01). 27-OHC augmented the differentiation of lung fibroblasts into myofibroblasts and the production of extracellular matrix protein through activation of nuclear factor-kappa B and subsequent transforming growth factor-β(1) up-regulation. CONCLUSIONS:27-OHC production was enhanced in the airways of COPD patients and might be involved in the pathogenesis of COPD. |
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Authors:
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Takashi Kikuchi; Hisatoshi Sugiura; Akira Koarai; Tomohiro Ichikawa; Yoshiaki Minakata; Kazuto Matsunaga; Masanori Nakanishi; Tsunahiko Hirano; Keiichirou Akamatsu; Satoru Yanagisawa; Kanako Furukawa; Hiroki Kawabata; Masakazu Ichinose |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-26 |
Journal Detail:
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Title: Chest Volume: - ISSN: 1931-3543 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0231335 Medline TA: Chest Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Third Department of Internal Medicine, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama, Wakayama 641-0012, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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