| Incorporation of a sequential BMP-2/BMP-7 delivery system into chitosan-based scaffolds for bone tissue engineering. | |
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MedLine Citation:
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PMID: 19361857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to develop a 3-D construct carrying an inherent sequential growth factor delivery system. Poly(lactic acid-co-glycolic acid) (PLGA) nanocapsules loaded with bone morphogenetic protein BMP-2 and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanocapsules loaded with BMP-7 made the early release of BMP-2 and longer term release of BMP-7 possible. 3-D fiber mesh scaffolds were prepared from chitosan and from chitosan-PEO by wet spinning. Chitosan of 4% concentration in 2% acetic acid (CHI4-HAc2) and chitosan (4%) and PEO (2%) in 5% acetic acid (CHI4-PEO2-HAc5) yielded scaffolds with smooth and rough fiber surfaces, respectively. These scaffolds were seeded with rat bone marrow mesenchymal stem cells (MSCs). When there were no nanoparticles the initial differentiation rate was higher on (CHI4-HAc2) scaffolds but by three weeks both the scaffolds had similar alkaline phosphatase (ALP) levels. The cell numbers were also comparable by the end of the third week. Incorporation of nanoparticles into the scaffolds was achieved by two different methods: incorporation within the scaffold fibers (NP-IN) and on the fibers (NP-ON). It was shown that incorporation on the CHI4-HAc2 fibers (NP-ON) prevented the burst release observed with the free nanoparticles, but this did not influence the total amount released in 25 days. However NP-IN for the same fibers revealed a much slower rate of release; ca. 70% released at the end of incubation period. The effect of single, simultaneous and sequential delivery of BMP-2 and BMP-7 from the CHI4-HAc2 scaffolds was studied in vitro using samples prepared with both incorporation methods. The effect of delivered agents was higher with the NP-ON samples. Delivery of BMP-2 alone suppressed cell proliferation while providing higher ALP activity compared to BMP-7. Simultaneous delivery was not particularly effective on cell numbers and ALP activity. The sequential delivery of BMP-2 and BMP-7, on the other hand, led to the highest ALP activity per cell (while suppressing proliferation) indicating the synergistic effect of using both growth factors holds promise for the production of tissue engineered bone. |
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Authors:
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Pinar Yilgor; Kadriye Tuzlakoglu; Rui L Reis; Nesrin Hasirci; Vasif Hasirci |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-09 |
Journal Detail:
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Title: Biomaterials Volume: 30 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-05-25 Completed Date: 2009-07-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 3551-9 Citation Subset: IM |
Affiliation:
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METU, BIOMAT, Department of Biotechnology, 06531 Ankara, Turkey. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials / adverse effects, chemistry* Bone Morphogenetic Protein 2 / chemistry* Bone Morphogenetic Protein 7 / chemistry*, pharmacology Cell Proliferation / drug effects Cell Survival / drug effects Cells, Cultured Chitosan / chemistry* Lactic Acid / chemistry, pharmacology Male Mesenchymal Stem Cells / cytology, drug effects, ultrastructure Microscopy, Electron, Scanning Polyglycolic Acid / chemistry, pharmacology Rats Rats, Sprague-Dawley Tissue Engineering / methods* X-Ray Microtomography |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Bone Morphogenetic Protein 2; 0/Bone Morphogenetic Protein 7; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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