Document Detail

Incorporation of sciadonic acid into cellular phospholipids reduces pro-inflammatory mediators in murine macrophages through NF-κB and MAPK signaling pathways.
MedLine Citation:
PMID:  22889893     Owner:  NLM     Status:  Publisher    
Sciadonic acid (SCA; Δ5,11,14-20:3), a non-methylene-interrupted polyunsaturated fatty acid (NMIFA), can substitute for arachidonic acid (AA) and reduce prostaglandin E(2) (PGE(2)) synthesis in macrophages. However, little is known about how SCA exerts its anti-inflammatory effects. The objectives of this study were to purify SCA from seeds of Podocarpus nagi and investigate mechanisms underlying the modulatory effects of SCA on inflammatory responses in murine RAW264.7 macrophages. We describe how high-purity SCA (>98%) can be obtained using argentated column chromatography. SCA was dose-dependently incorporated into cellular phospholipids, and increasing SCA incorporation correlated with decreases in the proportions of AA, total monounsaturated fatty acids (MUFA) and total saturated fatty acids (SFA). SCA decreased production of PGE(2) (29%), nitric oxide (NO) (31%), interleukin-6 (IL-6) (34%) and tumor necrosis factor-α (TNF-α) (14%). The suppression of pro-inflammatory mediators was due, in part, to decreased expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). SCA incorporation suppressed nuclear factor-kappa B (NF-κB) translocation and phosphorylation of mitogen-activated protein kinases (MAPK), including extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK). These findings indicate that by altering the cellular fatty acid composition SCA can modulate the responsiveness of macrophages to LPS through inactivation of NF-κB and MAPK pathways.
Szu-Jung Chen; Wen-Cheng Huang; Tzu-Ting Yang; Jui-Hua Lu; Lu-Te Chuang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-6
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  -     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Department of Radiation Oncology, Tao Yuan General Hospital, Tao-Yuan, Taiwan.
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