| Incorporation of radioiodinated fatty acids into cardiac phospholipids of normoxic canine myocardium. | |
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MedLine Citation:
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PMID: 1480157 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to assess the phospholipid distribution of radioiodinated 17-iodoheptadecanoic acid (IHDA), 15-(p-iodophenyl)pentadecanoic acid (p-IPPA) and 15-(p-iodophenyl)-3,3-dimethylpentadecanoic acid (DMIPPA) under normoxic conditions and to compare these data with the fatty acid composition of the phospholipid classes. After simultaneous i.v. injection of the radioiodinated fatty acids (I-123-IHDA; I-131-p-IPPA; I-125-DMIPPA) in open-chest dogs seven myocardial biopsies were taken over 40 min (n = 26). After lipid extraction of the biopsies the organic phase was analyzed for both neutral and polar lipids by two different TLC systems. The following polar lipid fractions were analyzed: lysophosphatidylcholine (LPC), sphingomyelin (SPH), phosphatidylcholine (PC; lecithin), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG; cardiolipin) and neutral lipids. Fractions were counted in a gamma well counter and corrected for cross-over and recovery. Results of the polar phospholipids analysis showed that IHDA has the highest incorporation into the phospholipids. The IHDA was mainly incorporated into PI (45.6%) followed by PC (30.9%), PE (14.0%) and PS (5.6%). The p-IPPA was predominantly incorporated incorporated into PC (37.2%), followed by PS (20.1%) and PE (13.7%). In contrast to IHDA, incorporation of p-IPPA into PI was small (6.4%). The DMIPPA analogue was incorporated into phospholipids to only a very small degree, compared to IHDA and p-IPPA.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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G W Sloof; F C Visser; T Teerlink; E F Comans; M J Eenige van; G J van der Vusse; F F Knapp |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 116 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1993-02-05 Completed Date: 1993-02-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 79-87 Citation Subset: IM |
Affiliation:
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Dept. of Cardiology, Metabolic Laboratory, Free University Hospital Amsterdam, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dogs Fatty Acids / metabolism*, pharmacokinetics, pharmacology Hemodynamics / drug effects Iodobenzenes / pharmacokinetics Male Membrane Lipids / metabolism Myocardium / metabolism* Oxygen / metabolism Phospholipids / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Iodobenzenes; 0/Membrane Lipids; 0/Phospholipids; 104426-56-4/15-(4-iodophenyl)-3,3-dimethylpentadecanoic acid; 63556-27-4/17-iodoheptadecanoic acid; 7782-44-7/Oxygen; 80479-93-2/omega-(4-iodophenyl)pentadecanoic acid |
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