Document Detail


Incorporation of radioiodinated IPPA and BMIPP fatty acid analogues into complex lipids from isolated rat hearts.
MedLine Citation:
PMID:  1629017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Heart lipids were extracted by the Folch technique from Langendorff-perfused rat hearts after administration of 15-(p-[131I]iodophenyl)pentadecanoic acid and 15-(p-[125I]iodophenyl)-3-R,S-methylpentadecanoic acid. Techniques utilizing successive high performance liquid chromatographic (HPLC) analyses have been developed for the evaluation of the uptake of the tracers into neutral lipids and phospholipids of the rat hearts. Phospholipids were separated on a SiO2 column eluted with a gradient of acetonitrile/water (97.5/2.5) and acetonitrile/water (85/15) followed by separation of the neutral lipids on a C-18 reversed phase column with a gradient consisting of acetonitrile and 2-propanol/hexane (60/40) containing 1 N H2SO4 (5 microL/100 mL). Both tracers show the incorporation into the expected major lipid classes.
Authors:
J Kropp; K R Ambrose; F F Knapp; H P Nissen; H J Biersack
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology     Volume:  19     ISSN:  0883-2897     ISO Abbreviation:  Int J Rad Appl Instrum B     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-08-18     Completed Date:  1992-08-18     Revised Date:  2008-02-21    
Medline Journal Info:
Nlm Unique ID:  8611098     Medline TA:  Int J Rad Appl Instrum B     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  283-8     Citation Subset:  IM    
Affiliation:
Department of Nuclear Medicine and University of Bonn, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chromatography, High Pressure Liquid
Fatty Acids / chemistry,  metabolism*
Female
Iodine Radioisotopes
Iodobenzenes / metabolism*
Isotope Labeling
Lipid Metabolism*
Lipids / chemistry
Myocardium / chemistry,  metabolism*
Perfusion
Rats
Rats, Inbred F344
Grant Support
ID/Acronym/Agency:
HL35500/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Iodine Radioisotopes; 0/Iodobenzenes; 0/Lipids; 116754-87-1/iodofiltic acid; 80479-93-2/omega-(4-iodophenyl)pentadecanoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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