| Incorporation into the tissues and turnover of arachidonic acid after administration to normal and essential fatty acid deficient rats. | |
| | |
MedLine Citation:
|
PMID: 3937159 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A comparison was made of the incorporation into the tissues and metabolism of [1-14C] arachidonic acid (AA) after i.v. administration to normal and EFA-deficient rats. At different times, ultra-thin whole body sections were prepared and the distribution of the radioactivity determined by autoradiograms. After 5 min, a considerable incorporation occurs in the following organs: subcutaneous and perispinal fat, liver, heart muscle, kidney and adrenal. The EFA deficient rats show a similar distribution but the radioactivity is longer retained. The total amount of radioactivity in the heart, liver, kidney and adrenal was measured at different times. A decline occurs in the heart, and an increase in the adrenal. In the urine, the highest amount of radioactivity is excreted on the first day. The excretion is lower in the EFA-deficient rats. Small amounts of radioactive metabolites with the chromatographic characteristics of PGE2 and 13,14 dihydro-15ketoPGE2 were isolated from urine. The amounts of 14CO2 produced were determined after the administration of [1-14C] AA. Half times were: 39 +/- 2.9 min in the EFA-deficient and 28 +/- 2.8 min in the normal rats. In the heart, AA is incorporated into phospholipids and neutral lipids. The following percentages were determined: phosphatidylinositol: 6.9 +/- 0.6%, phosphatidylcholine: 44 +/- 4.1%, phosphatidylethanolamine: 10.0 +/- 1.0%, neutral lipids: 9.3 +/- 1.6%. Several explanations can be given for the higher requirements of some tissues for AA. It could be, that this substance is used in the formation of particular membranes with a high AA content. Differences in the amounts of metabolites produced may also play a role. |
| | |
Authors:
|
F J Zijlstra; J E Vincent |
Related Documents
:
|
8301669 - Angiotensin ii receptor subtypes and biological responses in the rat heart. 19616109 - Qa interval as an indirect measure of cardiac contractility in the conscious telemeteri... 6198929 - Enhanced atp and gtp synthesis from hypoxanthine or inosine after myocardial ischemia. 15791049 - Decreased vagal control over heart rate in rats with right-sided congestive heart failu... 1523169 - Hemodynamic effects of calcitonin in the normal rat. 8637189 - Developmental differences in distribution of cyclic nucleotide phosphodiesterase isofor... 2126429 - Effect of ageing on adenylate cyclase activity and g-proteins in rat submandibular sali... 21153279 - Rat placental lactogen-i abolishes nocturnal prolactin surges in the pregnant rat. 10870079 - Fetuin in the developing neocortex of the rat: distribution and origin. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Prostaglandins, leukotrienes, and medicine Volume: 20 ISSN: 0262-1746 ISO Abbreviation: Prostaglandins Leukot Med Publication Date: 1985 Dec |
Date Detail:
|
Created Date: 1986-02-24 Completed Date: 1986-02-24 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8206868 Medline TA: Prostaglandins Leukot Med Country: SCOTLAND |
Other Details:
|
Languages: eng Pagination: 287-97 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arachidonic Acid Arachidonic Acids / administration & dosage, metabolism* Autoradiography Carbon Dioxide / analysis Carbon Radioisotopes Fatty Acids, Essential / deficiency* Injections, Intravenous Kinetics Male Rats Rats, Inbred Strains Tissue Distribution |
| Chemical | |
Reg. No./Substance:
|
0/Arachidonic Acids; 0/Carbon Radioisotopes; 0/Fatty Acids, Essential; 124-38-9/Carbon Dioxide; 506-32-1/Arachidonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Antitumor activity in a rat mammary adenocarcinoma: the effect of cyclooxygenase inhibitors and immu...
Next Document: Adrenergic stimulation of prostacyclin production in the rat tail artery. II. The role of calcium.