| Incorporation of an inducible nucleotide analog into DNA by DNA polymerases. | |
| | |
MedLine Citation:
|
PMID: 19286386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Non-natural nucleotides with diverse functionalities are highly useful in many areas of basic research and practical applications. We have previously developed an efficient method for post-synthetic modifications of 2-amino-6-vinylpurine (AVP)-containing oligonucleotides, which permits conjugations of a variety of useful functional appendages to the AVP moiety in DNA. Here we report an investigation on the ability of various DNA polymerases to use 5'-triphosphate of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine (a stable precursor of AVP) as the substrate for templated DNA synthesis. |
| | |
Authors:
|
Md Monsur Ali; Shuhei Imoto; Yingfu Li; Shigeki Sasaki; Fumi Nagatsugi |
Related Documents
:
|
11814346 - Disruption of hiv-1 integrase-dna complexes by short 6-oxocytosine-containing oligonucl... 3375076 - Synthesis and hybridization of a series of biotinylated oligonucleotides. 12694176 - Antisense technologies. improvement through novel chemical modifications. 11111036 - Combined molecular ecological and confocal laser scanning microscopic analysis of peat ... 15802246 - Dam methylation: coordinating cellular processes. 7190076 - Kinetic analysis of the in vitro binding of radioactive cis- and trans-dichlorodiammine... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-20 |
Journal Detail:
|
Title: Bioorganic & medicinal chemistry Volume: 17 ISSN: 1464-3391 ISO Abbreviation: Bioorg. Med. Chem. Publication Date: 2009 Apr |
Date Detail:
|
Created Date: 2009-03-30 Completed Date: 2009-06-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9413298 Medline TA: Bioorg Med Chem Country: England |
Other Details:
|
Languages: eng Pagination: 2859-63 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Canada ON L8N 3Z5. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence DNA-Directed DNA Polymerase / metabolism* Oligodeoxyribonucleotides / biosynthesis*, chemistry Purine Nucleotides / chemical synthesis*, chemistry Templates, Genetic |
| Chemical | |
Reg. No./Substance:
|
0/2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine nucleoside triphosphate; 0/Oligodeoxyribonucleotides; 0/Purine Nucleotides; EC 2.7.7.7/DNA-Directed DNA Polymerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Overnight switching from ergot-derived dopamine agonists to pramipexole in patients with Parkinson's...
Next Document: Nanoindentation of gold nanoparticles functionalized with proteins.