Document Detail

Incorporation of eicosapentaenoic and docosahexaenoic acids by a yeast (FO726A).
MedLine Citation:
PMID:  10945786     Owner:  NLM     Status:  MEDLINE    
An eicosapentaenoic acid (EPA)- and docosahexaenoic acid (DHA)-incorporating yeast, FO726A, was putatively identified as Candida guilliermondii on the basis of morphological, physiological and biochemical characteristics. Culture conditions for FO726A were investigated with respect to cell mass productivity, cellular accumulation of total lipid, triglyceride (TG), EPA and DHA. When grown at 20 degrees C for 24 h in an optimal medium containing 1 g scrap fish oil, the yeast yielded 820 mg dry cells which consisted of 40.7% lipid, 40.2% protein and 14.1% carbohydrate. The lipid (334mg) consisted of 300 mg TG (36.6% of dry cells), 23.2 mg EPA (2.8%) and 54.8 mg DHA (6.7%), and the recovery rates of EPA and DHA from the fish oil were 27.1 and 43.6%, respectively. The positional distributions of fatty acids in the TG from the yeast were then investigated and compared with those in the TG from the fish oil. The EPA and DHA in the fish oil were concentrated more in the sn-1,3 positions (8.8 and 13.7%, respectively) than in the sn-2 position (3.7 and 10.8%, respectively). In the case of the TG from the yeast, EPA was present to a greater extent in the sn-1,3 positions than in the sn-2 position. In contrast, DHA was preferentially present in the sn-2 position, approximately twice that in the sn-1,3 positions.
X Guo; Y Ota
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  89     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-11-07     Completed Date:  2000-11-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  107-15     Citation Subset:  IM    
Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University, Japan.
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MeSH Terms
Candida / classification,  growth & development,  metabolism*
Culture Media
Docosahexaenoic Acids / metabolism*
Eicosapentaenoic Acid / metabolism*
Fatty Acids / metabolism
Fish Oils / metabolism
Phospholipids / metabolism
Triglycerides / metabolism
Reg. No./Substance:
0/Culture Media; 0/Fatty Acids; 0/Fish Oils; 0/Phospholipids; 0/Triglycerides; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids

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