| Incorporation of eicosapentaenoic and docosahexaenoic acids by a yeast (FO726A). | |
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MedLine Citation:
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PMID: 10945786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An eicosapentaenoic acid (EPA)- and docosahexaenoic acid (DHA)-incorporating yeast, FO726A, was putatively identified as Candida guilliermondii on the basis of morphological, physiological and biochemical characteristics. Culture conditions for FO726A were investigated with respect to cell mass productivity, cellular accumulation of total lipid, triglyceride (TG), EPA and DHA. When grown at 20 degrees C for 24 h in an optimal medium containing 1 g scrap fish oil, the yeast yielded 820 mg dry cells which consisted of 40.7% lipid, 40.2% protein and 14.1% carbohydrate. The lipid (334mg) consisted of 300 mg TG (36.6% of dry cells), 23.2 mg EPA (2.8%) and 54.8 mg DHA (6.7%), and the recovery rates of EPA and DHA from the fish oil were 27.1 and 43.6%, respectively. The positional distributions of fatty acids in the TG from the yeast were then investigated and compared with those in the TG from the fish oil. The EPA and DHA in the fish oil were concentrated more in the sn-1,3 positions (8.8 and 13.7%, respectively) than in the sn-2 position (3.7 and 10.8%, respectively). In the case of the TG from the yeast, EPA was present to a greater extent in the sn-1,3 positions than in the sn-2 position. In contrast, DHA was preferentially present in the sn-2 position, approximately twice that in the sn-1,3 positions. |
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Authors:
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X Guo; Y Ota |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of applied microbiology Volume: 89 ISSN: 1364-5072 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-11-07 Completed Date: 2000-11-14 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 107-15 Citation Subset: IM |
Affiliation:
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Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University, Japan. xuguo33@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Candida
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classification,
growth & development,
metabolism* Culture Media Docosahexaenoic Acids / metabolism* Eicosapentaenoic Acid / metabolism* Fatty Acids / metabolism Fish Oils / metabolism Phospholipids / metabolism Temperature Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids; 0/Fish Oils; 0/Phospholipids; 0/Triglycerides; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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