| Incorporation, distribution, and turnover of arachidonic acid within membrane phospholipids of B220+ T cells from autoimmune-prone MRL-lpr/lpr mice. | |
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MedLine Citation:
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PMID: 2106567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metabolism of AA-containing phosphoglycerides within T cell membranes leads to the generation of second messengers that appear to play a crucial role in transmembrane signal transduction. To test the hypothesis that aberrations in the movement of arachidonoyl-phospholipids are associated with and may potentially contribute to abnormal T cell function, the incorporation, distribution, and turnover of AA within the membrane glycerolipids of cells that are known to exhibit immunoregulatory disturbances was examined. Thy-1+, Ly-1+, L3T4-, Lyt-2-, B220+ T cells from autoimmune MRL-lpr/lpr mice were used as the cellular model. In contrast to control lymph node T cells, which preferentially incorporate labeled AA into phosphatidylcholine (PC), B220+ T cells displayed a predilection for distributing [3H]arachidonate into phosphatidylinositol (PI). The arachidonoyl-phospholipid pools were normal in B220+ T cells. The constitutive turnover of [3H]arachidonoyl-PI was significantly enhanced and that of [3H]arachidonate-PC substantially reduced in B220+ T cell compared with control cells. Using membrane homogenates B220+ T cells demonstrated a functional increase in the levels of lyso-PI. Intact B220+ T cells prelabeled with [3H]myoinositol and cultured in the absence of stimulation with exogenous antigens or mitogens, exhibited increased production of lyso-PI. The data indicate that the preferential formation of [3H]arachidonoyl-PI in B220+ T cells is the result of greatly increased, constitutive PI turnover that appears to be due to a membrane phospholipase A2 activity. It remains possible that disturbances in the movement of arachidonate within phospholipids of B220+ T cells play a role in the expression of aberrant immunological activity. |
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Authors:
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M Tomita-Yamaguchi; J F Babich; R C Baker; T J Santoro |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of experimental medicine Volume: 171 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-04-12 Completed Date: 1990-04-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 787-800 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Colorado Health Sciences Center, Denver. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid Arachidonic Acids / metabolism* Autoimmune Diseases / metabolism* Lymph Nodes / metabolism Lymphoproliferative Disorders / metabolism Lysophospholipids / metabolism Membrane Lipids / metabolism* Mice Mice, Inbred Strains Phospholipases / analysis Phospholipids / metabolism* T-Lymphocytes / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AA-07157/AA/NIAAA NIH HHS; AI-26284/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Lysophospholipids; 0/Membrane Lipids; 0/Phospholipids; 0/lysophosphatidylinositol; 506-32-1/Arachidonic Acid; EC 3.1.-/Phospholipases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Exp Med ISSN: 0022-1007 ISSN: 1540-9538 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 3 Year: 1990 Volume: 171 Issue: 3 First Page: 787 Last Page: 800 ID: 2187770 Publisher Id: 90171844 PubMed Id: 2106567 |
| Incorporation, distribution, and turnover of arachidonic acid within membrane phospholipids of B220+ T cells from autoimmune-prone MRL- lpr/lpr mice | |
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