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Incorporation, distribution, and turnover of arachidonic acid within membrane phospholipids of B220+ T cells from autoimmune-prone MRL-lpr/lpr mice.
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MedLine Citation:
PMID:  2106567     Owner:  NLM     Status:  MEDLINE    
The metabolism of AA-containing phosphoglycerides within T cell membranes leads to the generation of second messengers that appear to play a crucial role in transmembrane signal transduction. To test the hypothesis that aberrations in the movement of arachidonoyl-phospholipids are associated with and may potentially contribute to abnormal T cell function, the incorporation, distribution, and turnover of AA within the membrane glycerolipids of cells that are known to exhibit immunoregulatory disturbances was examined. Thy-1+, Ly-1+, L3T4-, Lyt-2-, B220+ T cells from autoimmune MRL-lpr/lpr mice were used as the cellular model. In contrast to control lymph node T cells, which preferentially incorporate labeled AA into phosphatidylcholine (PC), B220+ T cells displayed a predilection for distributing [3H]arachidonate into phosphatidylinositol (PI). The arachidonoyl-phospholipid pools were normal in B220+ T cells. The constitutive turnover of [3H]arachidonoyl-PI was significantly enhanced and that of [3H]arachidonate-PC substantially reduced in B220+ T cell compared with control cells. Using membrane homogenates B220+ T cells demonstrated a functional increase in the levels of lyso-PI. Intact B220+ T cells prelabeled with [3H]myoinositol and cultured in the absence of stimulation with exogenous antigens or mitogens, exhibited increased production of lyso-PI. The data indicate that the preferential formation of [3H]arachidonoyl-PI in B220+ T cells is the result of greatly increased, constitutive PI turnover that appears to be due to a membrane phospholipase A2 activity. It remains possible that disturbances in the movement of arachidonate within phospholipids of B220+ T cells play a role in the expression of aberrant immunological activity.
M Tomita-Yamaguchi; J F Babich; R C Baker; T J Santoro
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  171     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-04-12     Completed Date:  1990-04-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  787-800     Citation Subset:  IM    
Department of Medicine, University of Colorado Health Sciences Center, Denver.
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MeSH Terms
Arachidonic Acid
Arachidonic Acids / metabolism*
Autoimmune Diseases / metabolism*
Lymph Nodes / metabolism
Lymphoproliferative Disorders / metabolism
Lysophospholipids / metabolism
Membrane Lipids / metabolism*
Mice, Inbred Strains
Phospholipases / analysis
Phospholipids / metabolism*
T-Lymphocytes / metabolism*
Grant Support
Reg. No./Substance:
0/Arachidonic Acids; 0/Lysophospholipids; 0/Membrane Lipids; 0/Phospholipids; 0/lysophosphatidylinositol; 506-32-1/Arachidonic Acid; EC 3.1.-/Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 3 Year: 1990
Volume: 171 Issue: 3
First Page: 787 Last Page: 800
ID: 2187770
Publisher Id: 90171844
PubMed Id: 2106567

Incorporation, distribution, and turnover of arachidonic acid within membrane phospholipids of B220+ T cells from autoimmune-prone MRL- lpr/lpr mice

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