| Incorporation of coenzyme M into component C of methylcoenzyme M methylreductase during in vitro methanogenesis. | |
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MedLine Citation:
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PMID: 3106338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reduction of the methyl group of [methyl-3H,thio-35S]2-methylthioethanesulfonic acid to methane by a reconstituted enzyme system resulted in a slow incorporation of [thio-35S]2-mercaptoethanesulfonic acid (HS-CoM) into component C of the methylreductase system. Only 35S label was associated with component C. The ratio of incorporated HS-CoM to component C was 1.96 to 1. The ratio of HS-CoM to factor F430, the nickel-containing cofactor of component C, was 1.18 to 1. Extraction of factor F430 from the protein resulted in the release of 62 +/- 8% of the 35S label, but the label was not covalently bound to F430. The incorporation of label into component C was coupled to methyl group reduction; no label was found associated with component C from a reconstituted reaction containing unlabeled 2-methylthioethanesulfonic acid and [thio-35S]HS-CoM. |
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Authors:
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P L Hartzell; M I Donnelly; R S Wolfe |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 262 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1987 Apr |
Date Detail:
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Created Date: 1987-06-05 Completed Date: 1987-06-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5581-6 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Euryarchaeota
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enzymology* Kinetics Mercaptoethanol / analogs & derivatives* Mesna / isolation & purification, metabolism* Methane / metabolism* Oxidoreductases / isolation & purification, metabolism* Sulfur Radioisotopes |
| Grant Support | |
ID/Acronym/Agency:
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AI 12277/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Sulfur Radioisotopes; 19767-45-4/Mesna; 60-24-2/Mercaptoethanol; 74-82-8/Methane; EC 1.-/Oxidoreductases; EC 2.8.4.1/methyl coenzyme M reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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