Document Detail

Incorporation of coenzyme M into component C of methylcoenzyme M methylreductase during in vitro methanogenesis.
MedLine Citation:
PMID:  3106338     Owner:  NLM     Status:  MEDLINE    
Reduction of the methyl group of [methyl-3H,thio-35S]2-methylthioethanesulfonic acid to methane by a reconstituted enzyme system resulted in a slow incorporation of [thio-35S]2-mercaptoethanesulfonic acid (HS-CoM) into component C of the methylreductase system. Only 35S label was associated with component C. The ratio of incorporated HS-CoM to component C was 1.96 to 1. The ratio of HS-CoM to factor F430, the nickel-containing cofactor of component C, was 1.18 to 1. Extraction of factor F430 from the protein resulted in the release of 62 +/- 8% of the 35S label, but the label was not covalently bound to F430. The incorporation of label into component C was coupled to methyl group reduction; no label was found associated with component C from a reconstituted reaction containing unlabeled 2-methylthioethanesulfonic acid and [thio-35S]HS-CoM.
P L Hartzell; M I Donnelly; R S Wolfe
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  262     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-06-05     Completed Date:  1987-06-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5581-6     Citation Subset:  IM    
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MeSH Terms
Euryarchaeota / enzymology*
Mercaptoethanol / analogs & derivatives*
Mesna / isolation & purification,  metabolism*
Methane / metabolism*
Oxidoreductases / isolation & purification,  metabolism*
Sulfur Radioisotopes
Grant Support
Reg. No./Substance:
0/Sulfur Radioisotopes; 19767-45-4/Mesna; 60-24-2/Mercaptoethanol; 74-82-8/Methane; EC 1.-/Oxidoreductases; EC coenzyme M reductase

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